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dc.contributor.authorLewis, Bonnie
dc.contributor.authorDiaz-Pulido, Guillermo
dc.date.accessioned2017-06-22T01:09:42Z
dc.date.available2017-06-22T01:09:42Z
dc.date.issued2017
dc.identifier.issn0022-0981
dc.identifier.doi10.1016/j.jembe.2017.02.004
dc.identifier.urihttp://hdl.handle.net/10072/340675
dc.description.abstractCoralline algae play a central role in reef ecology and have been identified as one of the most sensitive calcifying organisms to ocean acidification, and as such are potential indicators for evaluating the future effects of climate change. Despite their importance, little information is available on their growth and calcification rates in situ, highlighting the need for a more effective technique to obtain these measurements. In this study we compared two alternative fluorochrome markers (calcein and calcofluor white - CFW) against the commonly used alizarin red stain in order to establish a more efficient and effective staining protocol for use in the field on both branching (Lithophyllum pygmaeum) and encrusting (Porolithon onkodes) coralline species. Each marker was tested using three different concentration and immersion times for toxicity, visibility and efficiency. Results from this study found that while alizarin displayed a high visibility frequency it also reduced growth in the branching species. The CFW staining proved unreliable for the encrusting samples with usable marks in only 3 of 9 treatments and also reduced growth in branching treatments. Calcein, however, proved to be an effective and efficient marker for in situ coralline studies with a high visibility frequency, no toxic effects on growth and a short immersion time of 3 h or less. This identification of a more efficient and effective stain to use in situ on coralline algae can potentially improve demographic studies in both the field and laboratory environments, further facilitating future climate change research.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier Science
dc.relation.ispartofpagefrom64
dc.relation.ispartofpageto73
dc.relation.ispartofjournalJournal of Experimental Marine Biology and Ecology
dc.relation.ispartofvolume490
dc.subject.fieldofresearchEnvironmental Sciences not elsewhere classified
dc.subject.fieldofresearchEnvironmental Sciences
dc.subject.fieldofresearchBiological Sciences
dc.subject.fieldofresearchAgricultural and Veterinary Sciences
dc.subject.fieldofresearchcode059999
dc.subject.fieldofresearchcode05
dc.subject.fieldofresearchcode06
dc.subject.fieldofresearchcode07
dc.titleSuitability of three fluorochrome markers for obtaining in situ growth rates of coralline algae
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.facultyGriffith Sciences, Griffith School of Environment
gro.hasfulltextNo Full Text
gro.griffith.authorDiaz-Pulido, Guillermo
gro.griffith.authorLewis, Bonnie E.


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