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dc.contributor.authorTang, Jun
dc.contributor.authorK. Wang, Conan
dc.contributor.authorPan, Xulin
dc.contributor.authorYan, He
dc.contributor.authorZeng, Guangzhi
dc.contributor.authorXu, Wenyan
dc.contributor.authorHe, Wenjun
dc.contributor.authorDaly, Norelle L.
dc.contributor.authorJ. Craik, David
dc.contributor.authorTan, Ninghua
dc.date.accessioned2017-05-03T15:40:23Z
dc.date.available2017-05-03T15:40:23Z
dc.date.issued2010
dc.date.modified2010-09-17T07:07:04Z
dc.identifier.issn01969781
dc.identifier.doi10.1016/j.peptides.2010.05.004
dc.identifier.urihttp://hdl.handle.net/10072/34094
dc.description.abstractMany plants of the Violaceae plant family have been used in traditional remedies, and these plants often contain cyclotides, a particular type of plant cyclopeptide that is distinguished by a cyclic cystine knot motif. In general, bioactive plant cyclopeptides are interesting candidates for drug development. In the current study, a suite of 14 cyclotides, which includes seven novel cyclotides [vitri B, C, D, E, F, varv Hm, and He], together with seven known cyclotides [varv A, D, E, F, H, vitri A, and cycloviolacin O2], was isolated from Viola tricolor, a common flower. A chromatography-based method was used to isolate the cyclotides, which were characterized using tandem mass spectrometry and NMR spectroscopy. Several of the cyclotides showed cytotoxic activities against five cancer cell lines, U251, MDA-MB-231, A549, DU145, and BEL-7402. Three cyclotides, vitri A, vitri F, and cycloviolacin O2, were the most cytotoxic. The cytotoxic activity of the cyclotides did not correlate well with their hemolytic activity, indicating that different interactions, most likely with membranes, are involved for cytotoxic and hemolytic activities. Homology modeling of the structures was used in deriving structure-activity relationships.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier
dc.publisher.placeUnited States
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom1434
dc.relation.ispartofpageto1440
dc.relation.ispartofissue3
dc.relation.ispartofjournalPeptides
dc.relation.ispartofvolume31
dc.rights.retentionY
dc.subject.fieldofresearchStructural Biology (incl. Macromolecular Modelling)
dc.subject.fieldofresearchMedicinal and Biomolecular Chemistry
dc.subject.fieldofresearchPhysiology
dc.subject.fieldofresearchPharmacology and Pharmaceutical Sciences
dc.subject.fieldofresearchcode060112
dc.subject.fieldofresearchcode0304
dc.subject.fieldofresearchcode0606
dc.subject.fieldofresearchcode1115
dc.titleIsolation and characterization of cytotoxic cyclotides from Viola tricolor
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.date.issued2010
gro.hasfulltextNo Full Text
gro.griffith.authorWang, Conan K.


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