Extracellular polysaccharide from Ralstonia solanacearum; A strong inducer of eggplant defense against bacterial wilt
Author(s)
Prakasha, Avinash
Grice, I Darren
Kumar, KS Vinay
Sadashiva, MP
Shankar, HN
Umesha, S
Griffith University Author(s)
Year published
2017
Metadata
Show full item recordAbstract
Ralstonia solanacearum a phytopathogenic bacterium produces virulence factor via production of extracellular polysaccharides (EPS). We have isolated EPS from R. solanacearum and characterized this by AFM, SEM, FTIR, LC-MS and NMR spectroscopy. Interestingly, the result showed pore size distribution, topology, rheological and pseudoplastic behaviour of EPS and three major monosaccharides, glucuronic acid, N-acetylglucosamine and glucose associated with EPS. Eggplant treated with intact crude EPS showed a significant 51% decrease in bacterial wilt incidence for both in vitro and greenhouse conditions. In eggplant cultivars EPS ...
View more >Ralstonia solanacearum a phytopathogenic bacterium produces virulence factor via production of extracellular polysaccharides (EPS). We have isolated EPS from R. solanacearum and characterized this by AFM, SEM, FTIR, LC-MS and NMR spectroscopy. Interestingly, the result showed pore size distribution, topology, rheological and pseudoplastic behaviour of EPS and three major monosaccharides, glucuronic acid, N-acetylglucosamine and glucose associated with EPS. Eggplant treated with intact crude EPS showed a significant 51% decrease in bacterial wilt incidence for both in vitro and greenhouse conditions. In eggplant cultivars EPS induces significant up-regulation of guaiacol peroxidase and ascorbate peroxidase defense genes, providing screen resistance against bacterial wilt.
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View more >Ralstonia solanacearum a phytopathogenic bacterium produces virulence factor via production of extracellular polysaccharides (EPS). We have isolated EPS from R. solanacearum and characterized this by AFM, SEM, FTIR, LC-MS and NMR spectroscopy. Interestingly, the result showed pore size distribution, topology, rheological and pseudoplastic behaviour of EPS and three major monosaccharides, glucuronic acid, N-acetylglucosamine and glucose associated with EPS. Eggplant treated with intact crude EPS showed a significant 51% decrease in bacterial wilt incidence for both in vitro and greenhouse conditions. In eggplant cultivars EPS induces significant up-regulation of guaiacol peroxidase and ascorbate peroxidase defense genes, providing screen resistance against bacterial wilt.
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Journal Title
Biological Control
Volume
110
Subject
Zoology
Zoology not elsewhere classified