Show simple item record

dc.contributor.authorBriffa, Jessica F
dc.contributor.authorGrinfeld, Esther
dc.contributor.authorMathai, Michael L
dc.contributor.authorPoronnik, Phillip
dc.contributor.authorMcAinch, Andrew J
dc.contributor.authorHryciw, Deanne H
dc.date.accessioned2017-07-17T05:00:14Z
dc.date.available2017-07-17T05:00:14Z
dc.date.issued2015
dc.identifier.issn0303-7207
dc.identifier.doi10.1016/j.mce.2014.11.024
dc.identifier.urihttp://hdl.handle.net/10072/341912
dc.description.abstractIncreased leptin concentrations observed in obesity can lead to proteinuria, suggesting that leptin may play a role in obesity-related kidney disease. Obesity reduces activation of AMP-activated protein kinase (AMPK) and increases transforming growth factor-β1 (TGF-β1) expression in the kidney, leading to albuminuria. Thus we investigated if elevated leptin altered AMPK and TGF-β1 signaling in proximal tubule cells (PTCs). In opossum kidney (OK) PTCs Western blot analysis demonstrated that leptin upregulates TGF-β1 secretion (0.50 µg/ml) and phosphorylated AMPKα (at 0.25, and 0.50 µg/ml), and downregulates megalin expression at all concentrations (0.05–0.50 µg/ml). Using the AMPK inhibitor, Compound C, leptin exposure regulated TGF-β1 expression and secretion in PTCs via an AMPK mediated pathway. In addition, elevated leptin exposure (0.50 µg/ml) reduced albumin handling in OK cells independently of megalin expression. This study demonstrates that leptin upregulates TGF-β1, reduces megalin, and reduces albumin handling in PTCs by an AMPK mediated pathway.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier
dc.relation.ispartofpagefrom25
dc.relation.ispartofpageto34
dc.relation.ispartofjournalMolecular and Cellular Endocrinology
dc.relation.ispartofvolume401
dc.subject.fieldofresearchMedical Physiology not elsewhere classified
dc.subject.fieldofresearchBiological Sciences
dc.subject.fieldofresearchAgricultural and Veterinary Sciences
dc.subject.fieldofresearchMedical and Health Sciences
dc.subject.fieldofresearchcode111699
dc.subject.fieldofresearchcode06
dc.subject.fieldofresearchcode07
dc.subject.fieldofresearchcode11
dc.titleAcute leptin exposure reduces megalin expression and upregulates TGFB1 in cultured renal proximal tubule cells
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.hasfulltextNo Full Text
gro.griffith.authorSkelly, Deanne


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record