dc.contributor.author | Cornall, Lauren M | |
dc.contributor.author | Mathai, Michael L | |
dc.contributor.author | Hryciw, Deanne H | |
dc.contributor.author | McAinch, Andrew J | |
dc.date.accessioned | 2017-07-17T05:55:35Z | |
dc.date.available | 2017-07-17T05:55:35Z | |
dc.date.issued | 2011 | |
dc.identifier.issn | 1015-8987 | |
dc.identifier.doi | 10.1159/000335820 | |
dc.identifier.uri | http://hdl.handle.net/10072/341928 | |
dc.description.abstract | Background/Aims: GPR43 and GPR120 have recently been deorphanised as receptors for fatty acids. Fatty acids mediate a variety of metabolic processes in the body, however, the effect these receptors have on metabolism is not fully understood. Here, we characterise the effect of diet-induced obesity on the expression of GPR43 and GPR120 in tissues important in maintaining metabolic health. Methods: Six-week old male Sprague Dawley rats were fed either a high fat diet (HFD; 22% fat) or control diet (5% fat; n = 8-9/group) for 12 weeks. Rats were euthanized and the heart, liver, soleus and extensor digitorum longus (EDL) skeletal muscles were excised. GPR43 and GPR120 receptor abundance was quantified by ‘real-time’ PCR. Results: GPR43 mRNA abundance was significantly up-regulated by a HFD in liver and soleus and EDL skeletal muscles compared to control (p ≤ 0.05). Whilst a HFD significantly up-regulated GPR120 gene transcripts in cardiac tissue and EDL skeletal muscle when compare to control (p ≤ 0.05). Conclusion: We have shown for the first time that up-regulation of GPR43 and GPR120 in response to a HFD, is tissue specific. This suggests these receptors have different roles in mediating metabolic function in a number of tissues in the human body. | |
dc.description.peerreviewed | Yes | |
dc.language | English | |
dc.language.iso | eng | |
dc.publisher | S. Karger AG | |
dc.relation.ispartofpagefrom | 949 | |
dc.relation.ispartofpageto | 958 | |
dc.relation.ispartofissue | 5 | |
dc.relation.ispartofjournal | Cellular Physiology and Biochemistry | |
dc.relation.ispartofvolume | 28 | |
dc.subject.fieldofresearch | Biochemistry and cell biology | |
dc.subject.fieldofresearch | Medical physiology | |
dc.subject.fieldofresearch | Medical physiology not elsewhere classified | |
dc.subject.fieldofresearchcode | 3101 | |
dc.subject.fieldofresearchcode | 3208 | |
dc.subject.fieldofresearchcode | 320899 | |
dc.title | Diet-induced obesity up-regulates the abundance of GPR43 and GPR120 in a tissue specific manner | |
dc.type | Journal article | |
dc.type.description | C1 - Articles | |
dc.type.code | C - Journal Articles | |
gro.hasfulltext | No Full Text | |
gro.griffith.author | Skelly, Deanne | |