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  • A novel immuno-polymerase chain reaction protocol incorporating a highly purified streptavidin-DNA conjugate

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    Author(s)
    Shan, Jianguo
    Toye, Philip
    Griffith University Author(s)
    Shan, Jianguo
    Year published
    2009
    Metadata
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    Abstract
    We have developed an immuno-polymerase chain reaction protocol which includes a highly purified streptavidin-DNA conjugate. The protocol comprises standard ELISA methodology and washing buffers together with a real-time PCR read-out system. The conjugate was employed in both indirect and capture assay formats, which can be completed in a single day using standard laboratory equipment. The immuno-PCR is reproducible, with a larger dynamic range and a sensitivity several orders of magnitude greater than the corresponding conventional ELISA. The minimum concentration of analyte detected was of the order of 1 pg=mL These ...
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    We have developed an immuno-polymerase chain reaction protocol which includes a highly purified streptavidin-DNA conjugate. The protocol comprises standard ELISA methodology and washing buffers together with a real-time PCR read-out system. The conjugate was employed in both indirect and capture assay formats, which can be completed in a single day using standard laboratory equipment. The immuno-PCR is reproducible, with a larger dynamic range and a sensitivity several orders of magnitude greater than the corresponding conventional ELISA. The minimum concentration of analyte detected was of the order of 1 pg=mL These characteristics should contribute to the adoption of immuno-PCR by research and clinical laboratories.
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    Journal Title
    Journal of Immunoassay & Immunochemistry
    Volume
    30
    Issue
    3
    DOI
    https://doi.org/10.1080/15321810903084764
    Copyright Statement
    © 2009 Taylor & Francis. This is an electronic version of an article published in Journal of Immunoassay and Immunochemistry Volume 30, Issue 3, 2009, 322-337. Journal of Immunoassay and Immunochemistry is available online at: http://www.informaworld.com with the open URL of your article.
    Subject
    Humoural Immunology and Immunochemistry
    Immunology
    Publication URI
    http://hdl.handle.net/10072/34201
    Collection
    • Journal articles

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