A novel immuno-polymerase chain reaction protocol incorporating a highly purified streptavidin-DNA conjugate
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We have developed an immuno-polymerase chain reaction protocol which includes a highly purified streptavidin-DNA conjugate. The protocol comprises standard ELISA methodology and washing buffers together with a real-time PCR read-out system. The conjugate was employed in both indirect and capture assay formats, which can be completed in a single day using standard laboratory equipment. The immuno-PCR is reproducible, with a larger dynamic range and a sensitivity several orders of magnitude greater than the corresponding conventional ELISA. The minimum concentration of analyte detected was of the order of 1 pg=mL These characteristics should contribute to the adoption of immuno-PCR by research and clinical laboratories.
Journal of Immunoassay & Immunochemistry
Copyright 2009 Taylor & Francis. This is an electronic version of an article published in Journal of Immunoassay and Immunochemistry Volume 30, Issue 3, 2009, 322-337. Journal of Immunoassay and Immunochemistry is available online at: http://www.informaworld.com with the open URL of your article.
Humoural Immunology and Immunochemistry