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dc.contributor.authorXu, Shicai
dc.contributor.authorZhan, Jian
dc.contributor.authorMan, Baoyuan
dc.contributor.authorJiang, Shouzhen
dc.contributor.authorYue, Weiwei
dc.contributor.authorGao, Shoubao
dc.contributor.authorGuo, Chengang
dc.contributor.authorLiu, Hanping
dc.contributor.authorLi, Zhenhua
dc.contributor.authorWang, Jihua
dc.contributor.authorZhou, Yaoqi
dc.date.accessioned2017-07-27T04:47:13Z
dc.date.available2017-07-27T04:47:13Z
dc.date.issued2017
dc.identifier.issn2041-1723
dc.identifier.doi10.1038/ncomms14902
dc.identifier.urihttp://hdl.handle.net/10072/342668
dc.description.abstractReliable determination of binding kinetics and affinity of DNA hybridization and single-base mismatches plays an essential role in systems biology, personalized and precision medicine. The standard tools are optical-based sensors that are difficult to operate in low cost and to miniaturize for high-throughput measurement. Biosensors based on nanowire field-effect transistors have been developed, but reliable and cost-effective fabrication remains a challenge. Here, we demonstrate that a graphene single-crystal domain patterned into multiple channels can measure time- and concentration-dependent DNA hybridization kinetics and affinity reliably and sensitively, with a detection limit of 10 pM for DNA. It can distinguish single-base mutations quantitatively in real time. An analytical model is developed to estimate probe density, efficiency of hybridization and the maximum sensor response. The results suggest a promising future for cost-effective, high-throughput screening of drug candidates, genetic variations and disease biomarkers by using an integrated, miniaturized, all-electrical multiplexed, graphene-based DNA array.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherNature Publishing Group
dc.relation.ispartofpagefrom14902-1
dc.relation.ispartofpageto14902-10
dc.relation.ispartofjournalNature Communications
dc.relation.ispartofvolume8
dc.subject.fieldofresearchChemical Sciences not elsewhere classified
dc.subject.fieldofresearchcode039999
dc.titleReal-time reliable determination of binding kinetics of DNA hybridization using a multi-channel graphene biosensor
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttp://creativecommons.org/licenses/by/4.0/
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© The Author(s) 2017. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
gro.hasfulltextFull Text
gro.griffith.authorZhou, Yaoqi
gro.griffith.authorZhan, Jian


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