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dc.contributor.authorDraper, JL
dc.contributor.authorRehm, BH
dc.date.accessioned2017-07-28T02:46:25Z
dc.date.available2017-07-28T02:46:25Z
dc.date.issued2012
dc.identifier.issn1949-1018
dc.identifier.doi10.4161/bbug.19567
dc.identifier.urihttp://hdl.handle.net/10072/342765
dc.description.abstractThe ability to generate tailor-made, functionalized polyester (polyhydroxyalkanoate, PHA) beads in bacteria by harnessing their natural carbon-storage granule production system is an exciting recent development. Proteins that naturally attach to the polyester granule core were rationally engineered to enable in vivo production of PHA beads which are applicable in bioseparation, protein purification, enzyme immobilization and diagnostics and which show advantageous properties toward the development of safe and efficient particulate vaccines. These beads are recombinantly produced as fully functional, insoluble polyester inclusions that can be easily separated from the cell. This simple one-step production of functionalized beads provides a tantalizing alternative to current commercial functional beads, for which proteins must be expressed, purified and then chemically attached to solid supports. The recent success in generating antigen-displaying PHA granules in the food-grade bacterium Lactococcus lactis capable of mediating protective immunity against Mycobacterium tuberculosis infection highlights the promise and flexibility of this new technology.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherTaylor and Francis Inc
dc.relation.ispartofpagefrom203
dc.relation.ispartofpageto208
dc.relation.ispartofissue4
dc.relation.ispartofjournalBioengineered
dc.relation.ispartofvolume3
dc.subject.fieldofresearchMicrobiology not elsewhere classified
dc.subject.fieldofresearchGenetics
dc.subject.fieldofresearchMicrobiology
dc.subject.fieldofresearchcode060599
dc.subject.fieldofresearchcode0604
dc.subject.fieldofresearchcode0605
dc.titleEngineering bacteria to manufacture functionalized polyester beads
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.hasfulltextNo Full Text
gro.griffith.authorRehm, Bernd


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