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dc.contributor.authorHooks, David O
dc.contributor.authorRehm, Bernd HA
dc.date.accessioned2017-07-28T02:49:37Z
dc.date.available2017-07-28T02:49:37Z
dc.date.issued2015
dc.identifier.issn0141-5492
dc.identifier.doi10.1007/s10529-015-1803-7
dc.identifier.urihttp://hdl.handle.net/10072/342768
dc.description.abstractObjectives: To engineer a recombinant Escherichia coli to produce polyhydroxyalkanoate biopolymer beads displaying carbonic anhydrase (CA) from Desulfovibrio vulgaris str. “Miyazaki F” (DvCA). Results: The highest measured specific activity of the immobilised CA was 211 U/mg DvCA. The immobilised CA was thermostable, retaining 114 U/mg DvCA of activity after incubation at 90 °C for 1 h. Additionally, the immobilised CA tolerated 30 min incubations in a variety of pH conditions and was especially tolerant of alkaline conditions, retaining 131 U/mg DvCA of activity after pH 12 incubation. Conclusion: CA has a potential role in atmospheric CO2 mitigation strategies and the stability of the functionalised beads indicates suitability for use in industrial settings such as coal-fired power plants.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherSpringer Netherlands
dc.relation.ispartofpagefrom1415
dc.relation.ispartofpageto1420
dc.relation.ispartofissue7
dc.relation.ispartofjournalBiotechnology Letters
dc.relation.ispartofvolume37
dc.subject.fieldofresearchBiological sciences
dc.subject.fieldofresearchEngineering
dc.subject.fieldofresearchEnvironmental biotechnology not elsewhere classified
dc.subject.fieldofresearchcode31
dc.subject.fieldofresearchcode40
dc.subject.fieldofresearchcode410399
dc.titleSurface display of highly-stable Desulfovibrio vulgaris carbonic anhydrase on polyester beads for CO2 capture
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.hasfulltextNo Full Text
gro.griffith.authorRehm, Bernd


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