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dc.contributor.authorIslam, Farhadul
dc.contributor.authorGopalan, Vinod
dc.contributor.authorLaw, Simon
dc.contributor.authorTang, Johnny Cheuk-On
dc.contributor.authorChan, Kwok-Wah
dc.contributor.authorLam, Alfred King-Yin
dc.date.accessioned2017-08-07T01:07:55Z
dc.date.available2017-08-07T01:07:55Z
dc.date.issued2017
dc.identifier.issn0046-8177
dc.identifier.doi10.1016/j.humpath.2017.01.014
dc.identifier.urihttp://hdl.handle.net/10072/343055
dc.description.abstractMicroRNA-498 plays a crucial role in progression of many carcinomas. The signaling pathways by which miR-498 modulates carcinogenesis are still unknown. Also, miR-498–associated molecular pathogenesis has never been studied in esophageal squamous cell carcinoma (ESCC). Herein, we aimed to examine the expression and functional roles of miR-498 in ESCC as well as its influences on the clinicopathological features in patients with ESCC. Expression of miR-498 was investigated in 93 ESCC tissues and 5 ESCC cell lines using quantitative real-time polymerase chain reaction. In vitro effects of miR-498 on cellular process were studied followed by overexpression of miR-498. Western blot and immunofluorescence techniques were used to identify the interacting targets for miR-498 in ESCC. miR-498 expression was significantly reduced in ESCC when compared with the nonneoplastic esophageal tissues (P < .05). Patients with low miR-498 expression showed different histological grading of cancer and survival rates when compared with the patients with high miR-498 expression. Overexpression of miR-498 in ESCC cell lines induced remarkable reductions of cell proliferation, barrier penetration, and colony formation when compared with control and wild-type counterparts. Also, miR-498 activated the FOXO1/KLF6 transcriptional axis in ESCC. In addition, miR-498 overexpression increased p21 protein expression and led to reduced cancer cell growth. To conclude, reduced expression of miR-498 in ESCC and in vitro analysis have confirmed the tumor suppressor properties of miR-498 by modulating the FOXO1/KLF6 signaling pathway. The changes in miR-498 expression may have impacts on the clinical pathological parameters of ESCC as well as in the management of the patients with ESCC.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier
dc.relation.ispartofpagefrom141
dc.relation.ispartofpageto151
dc.relation.ispartofjournalHuman Pathology
dc.relation.ispartofvolume62
dc.subject.fieldofresearchClinical sciences
dc.subject.fieldofresearchPathology (excl. oral pathology)
dc.subject.fieldofresearchcode3202
dc.subject.fieldofresearchcode320220
dc.titleMiR-498 in esophageal squamous cell carcinoma: clinicopathological impacts and functional interactions
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttp://creativecommons.org/licenses/by/4.0/
dc.description.versionAccepted Manuscript (AM)
gro.facultyGriffith Health, School of Medicine
gro.rights.copyright© 2017 Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (http://creativecommons.org/licenses/by-nc-nd/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, providing that the work is properly cited.
gro.hasfulltextFull Text
gro.griffith.authorLam, Alfred K.
gro.griffith.authorGopalan, Vinod
gro.griffith.authorIslam, Farhad


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