dc.contributor.author | Islam, Farhadul | |
dc.contributor.author | Gopalan, Vinod | |
dc.contributor.author | Law, Simon | |
dc.contributor.author | Tang, Johnny Cheuk-On | |
dc.contributor.author | Chan, Kwok-Wah | |
dc.contributor.author | Lam, Alfred King-Yin | |
dc.date.accessioned | 2017-08-07T01:07:55Z | |
dc.date.available | 2017-08-07T01:07:55Z | |
dc.date.issued | 2017 | |
dc.identifier.issn | 0046-8177 | |
dc.identifier.doi | 10.1016/j.humpath.2017.01.014 | |
dc.identifier.uri | http://hdl.handle.net/10072/343055 | |
dc.description.abstract | MicroRNA-498 plays a crucial role in progression of many carcinomas. The signaling pathways by which miR-498 modulates carcinogenesis are still unknown. Also, miR-498–associated molecular pathogenesis has never been studied in esophageal squamous cell carcinoma (ESCC). Herein, we aimed to examine the expression and functional roles of miR-498 in ESCC as well as its influences on the clinicopathological features in patients with ESCC. Expression of miR-498 was investigated in 93 ESCC tissues and 5 ESCC cell lines using quantitative real-time polymerase chain reaction. In vitro effects of miR-498 on cellular process were studied followed by overexpression of miR-498. Western blot and immunofluorescence techniques were used to identify the interacting targets for miR-498 in ESCC. miR-498 expression was significantly reduced in ESCC when compared with the nonneoplastic esophageal tissues (P < .05). Patients with low miR-498 expression showed different histological grading of cancer and survival rates when compared with the patients with high miR-498 expression. Overexpression of miR-498 in ESCC cell lines induced remarkable reductions of cell proliferation, barrier penetration, and colony formation when compared with control and wild-type counterparts. Also, miR-498 activated the FOXO1/KLF6 transcriptional axis in ESCC. In addition, miR-498 overexpression increased p21 protein expression and led to reduced cancer cell growth. To conclude, reduced expression of miR-498 in ESCC and in vitro analysis have confirmed the tumor suppressor properties of miR-498 by modulating the FOXO1/KLF6 signaling pathway. The changes in miR-498 expression may have impacts on the clinical pathological parameters of ESCC as well as in the management of the patients with ESCC. | |
dc.description.peerreviewed | Yes | |
dc.language | English | |
dc.language.iso | eng | |
dc.publisher | Elsevier | |
dc.relation.ispartofpagefrom | 141 | |
dc.relation.ispartofpageto | 151 | |
dc.relation.ispartofjournal | Human Pathology | |
dc.relation.ispartofvolume | 62 | |
dc.subject.fieldofresearch | Clinical sciences | |
dc.subject.fieldofresearch | Pathology (excl. oral pathology) | |
dc.subject.fieldofresearchcode | 3202 | |
dc.subject.fieldofresearchcode | 320220 | |
dc.title | MiR-498 in esophageal squamous cell carcinoma: clinicopathological impacts and functional interactions | |
dc.type | Journal article | |
dc.type.description | C1 - Articles | |
dc.type.code | C - Journal Articles | |
dcterms.license | http://creativecommons.org/licenses/by/4.0/ | |
dc.description.version | Accepted Manuscript (AM) | |
gro.faculty | Griffith Health, School of Medicine | |
gro.rights.copyright | © 2017 Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (http://creativecommons.org/licenses/by-nc-nd/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, providing that the work is properly cited. | |
gro.hasfulltext | Full Text | |
gro.griffith.author | Lam, Alfred K. | |
gro.griffith.author | Gopalan, Vinod | |
gro.griffith.author | Islam, Farhad | |