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dc.contributor.authorBriggs, Matthew T
dc.contributor.authorHo, Yin Ying
dc.contributor.authorKaur, Gurjeet
dc.contributor.authorOehler, Martin K
dc.contributor.authorEverest-Dass, Arun V
dc.contributor.authorPacker, Nicolle H
dc.contributor.authorHoffmann, Peter
dc.date.accessioned2017-08-04T00:12:51Z
dc.date.available2017-08-04T00:12:51Z
dc.date.issued2017
dc.identifier.issn0951-4198
dc.identifier.doi10.1002/rcm.7845
dc.identifier.urihttp://hdl.handle.net/10072/343162
dc.description.abstractRationale: Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) of the proteome of a tissue has been an established technique for the past decade. In the last few years, MALDI-MSI of the N-glycome has emerged as a novel MALDI-MSI technique. To assess the accuracy and clinical significance of the N-linked glycan spatial distribution, we have developed a method that utilises MALDI-MSI followed by liquid chromatography coupled to tandem mass spectrometry (LC/MS/MS) in order to assign glycan structures to the differentiating MALDI-MSI glycan masses released from the tissue glycoproteins. Methods and Results: Our workflow presents a comprehensive list of instructions on how to (i) apply MALDI-MSI to spatially map the N-glycome across formalin-fixed paraffin-embedded (FFPE) clinical samples, (ii) structurally characterise N-glycans extracted from consecutive FFPE tissue sections by LC/MS/MS, and (iii) match relevant N-glycan masses from MALDI-MSI with confirmed N-glycan structures determined by LC/MS/MS. Conclusions: Our protocol provides groups that are new to this technique with instructions how to establish N-glycan MALDI-MSI in their laboratory. Furthermore, the method assigns N-glycan structural detail to the masses obtained in the MALDI-MS image.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherJohn Wiley & Sons Ltd.
dc.relation.ispartofpagefrom825
dc.relation.ispartofpageto841
dc.relation.ispartofissue10
dc.relation.ispartofjournalRapid Communications in Mass Spectrometry
dc.relation.ispartofvolume31
dc.subject.fieldofresearchAnalytical Chemistry not elsewhere classified
dc.subject.fieldofresearchChemical Sciences
dc.subject.fieldofresearchEarth Sciences
dc.subject.fieldofresearchBiological Sciences
dc.subject.fieldofresearchcode030199
dc.subject.fieldofresearchcode03
dc.subject.fieldofresearchcode04
dc.subject.fieldofresearchcode06
dc.titleN-Glycan matrix-assisted laser desorption/ionization mass spectrometry imaging protocol for formalin-fixed paraffin-embedded tissues
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.hasfulltextNo Full Text
gro.griffith.authorEverest-Dass, Arun
gro.griffith.authorPacker, Nicki


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