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  • A reversible fluorescence chemosensor for sequentially quantitative monitoring copper and sulfide in living cells

    Author(s)
    Meng, Qingtao
    Zhang, Run
    Jia, Hongmin
    Gao, Xue
    Wang, Cuiping
    Shi, Yu
    Everest-Dass, Arun V
    Zhang, Zhiqiang
    Griffith University Author(s)
    Everest-Dass, Arun
    Year published
    2015
    Metadata
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    Abstract
    We report a novel, selective and sensitive strategy for the sequentially “ON–OFF–ON” fluorescent detection of Cu2+ and S2− based on a fluorescein derivative, FL. The specific binding of FL towards Cu2+ in aqueous and biological media led to the intensive green fluorescence quenching and a notable increase of the absorbance maximum at 480 nm. In the presence of S2−, the intensity and overall pattern of the fluorescence emission and UV–vis spectra of FL–Cu2+ ensemble were recovered since the abolishment of paramagnetic Cu2+. This displacement approach exhibited highly specificity, and sensitivity with detection limits of 3 nM ...
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    We report a novel, selective and sensitive strategy for the sequentially “ON–OFF–ON” fluorescent detection of Cu2+ and S2− based on a fluorescein derivative, FL. The specific binding of FL towards Cu2+ in aqueous and biological media led to the intensive green fluorescence quenching and a notable increase of the absorbance maximum at 480 nm. In the presence of S2−, the intensity and overall pattern of the fluorescence emission and UV–vis spectra of FL–Cu2+ ensemble were recovered since the abolishment of paramagnetic Cu2+. This displacement approach exhibited highly specificity, and sensitivity with detection limits of 3 nM for Cu2+ and 150 nM for S2−. The fluorescence “ON–OFF–ON” circle can be repeated to a minimum of 5 times by the alternative addition of Cu2+ and S2−, implying that FL is a renewable dual-functional chemosensor. The biocompatibility of FL toward breast carcinoma cells, MDA-MB-231 was confirmed by MTT assay. The reversible “ON–OFF–ON” fluorescent response of FL to Cu2+ and S2− in living system was further confirmed by confocal fluorescence imaging of living cells. The quantification of Cu2+ and S2− in single intact cell was realized by the flow cytometry analysis.
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    Journal Title
    Talanta
    Volume
    143
    DOI
    https://doi.org/10.1016/j.talanta.2015.04.072
    Subject
    Analytical chemistry
    Analytical chemistry not elsewhere classified
    Other chemical sciences
    Publication URI
    http://hdl.handle.net/10072/343164
    Collection
    • Journal articles

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