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  • Electrochemical biosensing strategies for DNA methylation analysis

    Author(s)
    Hossain, Tanvir
    Mahmudunnabi, Golam
    Masud, Mostafa Kamal
    Islam, Md Nazmul
    Ooi, Lezanne
    Konstantinov, Konstantin
    Al Hossain, Md Shahriar
    Martinac, Boris
    Alici, Gursel
    Nguyen, Nam-Trung
    Shiddiky, Muhammad JA
    Griffith University Author(s)
    Nguyen, Nam-Trung
    Shiddiky, Muhammad J.
    Masud, Mostafa Kamal K.
    Islam, Nazmul
    Year published
    2017
    Metadata
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    Abstract
    DNA methylation is one of the key epigenetic modifications of DNA that results from the enzymatic addition of a methyl group at the fifth carbon of the cytosine base. It plays a crucial role in cellular development, genomic stability and gene expression. Aberrant DNA methylation is responsible for the pathogenesis of many diseases including cancers. Over the past several decades, many methodologies have been developed to detect DNA methylation. These methodologies range from classical molecular biology and optical approaches, such as bisulfite sequencing, microarrays, quantitative real-time PCR, colorimetry, Raman spectroscopy ...
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    DNA methylation is one of the key epigenetic modifications of DNA that results from the enzymatic addition of a methyl group at the fifth carbon of the cytosine base. It plays a crucial role in cellular development, genomic stability and gene expression. Aberrant DNA methylation is responsible for the pathogenesis of many diseases including cancers. Over the past several decades, many methodologies have been developed to detect DNA methylation. These methodologies range from classical molecular biology and optical approaches, such as bisulfite sequencing, microarrays, quantitative real-time PCR, colorimetry, Raman spectroscopy to the more recent electrochemical approaches. Among these, electrochemical approaches offer sensitive, simple, specific, rapid, and cost-effective analysis of DNA methylation. Additionally, electrochemical methods are highly amenable to miniaturization and possess the potential to be multiplexed. In recent years, several reviews have provided information on the detection strategies of DNA methylation. However, to date, there is no comprehensive evaluation of electrochemical DNA methylation detection strategies. Herein, we address the recent developments of electrochemical DNA methylation detection approaches. Furthermore, we highlight the major technical and biological challenges involved in these strategies and provide suggestions for the future direction of this important field.
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    Journal Title
    Biosensors and Bioelectronics
    Volume
    94
    DOI
    https://doi.org/10.1016/j.bios.2017.02.026
    Subject
    Analytical Chemistry not elsewhere classified
    Analytical Chemistry
    Biomedical Engineering
    Nanotechnology
    Publication URI
    http://hdl.handle.net/10072/343502
    Collection
    • Journal articles

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