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dc.contributor.authorFry, BN
dc.contributor.authorFeng, S
dc.contributor.authorChen, YY
dc.contributor.authorNewell, DG
dc.contributor.authorColoe, PJ
dc.contributor.authorKorolik, V
dc.date.accessioned2017-09-06T05:54:35Z
dc.date.available2017-09-06T05:54:35Z
dc.date.issued2000
dc.date.modified2007-04-02T05:15:39Z
dc.identifier.issn0019-9567
dc.identifier.doi10.1128/IAI.68.5.2594-2601.2000
dc.identifier.urihttp://hdl.handle.net/10072/3437
dc.description.abstractLipopolysaccharide (LPS) is one of the main virulence factors of gram-negative bacteria. The LPS from Campylobacter spp. has endotoxic properties and has been shown to play a role in adhesion. We previously cloned a gene cluster (wla) which is involved in the synthesis of the Campylobacter jejuni 81116 LPS molecule. Sequence alignment of the first gene in this cluster indicated similarity with galE genes. These genes encode a UDP-glucose 4-epimerase, which catalyzes the interconversion of UDP-galactose and UDP-glucose. A Salmonella galE mutant was transformed with the galE gene from C. jejuni. The LPS analysis of wild-type, galE, and complemented galE Salmonella strains showed that the C. jejuni galE gene could restore the smooth wild-type Salmonella LPS. A UDP-glucose 4-epimerase assay was used to demonstrate that the galE gene from C. jejuni encoded this epimerase. We constructed a C. jejuni galE mutant which expressed a lipid A-core molecule of reduced molecular weight that did not react with antiserum raised against the parental strain. These results show an essential role for the galE gene in the synthesis of C. jejuni LPS. The galE mutant also showed a reduction in its ability to adhere to and invade INT407 cells. However, it was still able to colonize chickens to the same level as the wild-type strain. The serum resistance and hemolytic activity of this mutant were not changed compared to the parent strain. The ability of the mutant to take up DNA and integrate it in its genome was reduced 20-fold. These results show that LPS of C. jejuni is an important virulence factor.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoen_AU
dc.publisherAmerican Society for Microbiology
dc.publisher.placeUSA
dc.relation.ispartofpagefrom2594
dc.relation.ispartofpageto2601
dc.relation.ispartofissue5
dc.relation.ispartofjournalInfection and Immunity
dc.relation.ispartofvolume68
dc.subject.fieldofresearchBiological Sciences
dc.subject.fieldofresearchAgricultural and Veterinary Sciences
dc.subject.fieldofresearchMedical and Health Sciences
dc.subject.fieldofresearchcode06
dc.subject.fieldofresearchcode07
dc.subject.fieldofresearchcode11
dc.titleThe galE Gene of Campylobacter jejuni Is Involved in Lipopolysaccharide Synthesis and Virulence
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dc.description.versionPublished
gro.facultyGriffith Sciences, Griffith Institute for Drug Discovery
gro.rights.copyright© 2000 American Society for Microbiology. The attached file is reproduced here in accordance with the copyright policy of the publisher. Please refer to the journal's website for access to the definitive, published version.
gro.date.issued2000
gro.hasfulltextFull Text
gro.griffith.authorKorolik, Victoria
gro.griffith.authorFry, Ben
gro.griffith.authorColoe, Peter
gro.griffith.authorFeng, Chien-Jung


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