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dc.contributor.authorLauer, FM
dc.contributor.authorKaemmerer, E
dc.contributor.authorMeckel, T
dc.date.accessioned2017-08-11T12:30:49Z
dc.date.available2017-08-11T12:30:49Z
dc.date.issued2014
dc.identifier.issn0169-409X
dc.identifier.doi10.1016/j.addr.2014.10.008
dc.identifier.urihttp://hdl.handle.net/10072/343833
dc.description.abstractFrom the onset of the first microscopic visualization of single fluorescent molecules in living cells at the beginning of this century, to the present, almost routine application of single molecule microscopy, the method has well-proven its ability to contribute unmatched detailed insight into the heterogeneous and dynamic molecular world life is composed of. Except for investigations on bacteria and yeast, almost the entire story of success is based on studies on adherent mammalian 2D cell cultures. However, despite this continuous progress, the technique was not able to keep pace with the move of the cell biology community to adapt 3D cell culture models for basic research, regenerative medicine, or drug development and screening. In this review, we will summarize the progress, which only recently allowed for the application of single molecule microscopy to 3D cell systems and give an overview of the technical advances that led to it. While initially posing a challenge, we finally conclude that relevant 3D cell models will become an integral part of the on-going success of single molecule microscopy.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier
dc.relation.ispartofpagefrom79
dc.relation.ispartofpageto94
dc.relation.ispartofjournalAdvanced Drug Delivery Reviews
dc.relation.ispartofvolume79
dc.subject.fieldofresearchPharmacology and Pharmaceutical Sciences not elsewhere classified
dc.subject.fieldofresearchPharmacology and Pharmaceutical Sciences
dc.subject.fieldofresearchcode111599
dc.subject.fieldofresearchcode1115
dc.titleSingle molecule microscopy in 3D cell cultures and tissues
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.hasfulltextNo Full Text
gro.griffith.authorKaemmerer, Elke


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