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dc.contributor.authorLoessner, Daniela
dc.contributor.authorMeinert, Christoph
dc.contributor.authorKaemmerer, Elke
dc.contributor.authorMartine, Laure C
dc.contributor.authorYue, Kan
dc.contributor.authorLevett, Peter A
dc.contributor.authorKlein, Travis J
dc.contributor.authorMelchels, Ferry PW
dc.contributor.authorKhademhosseini, Ali
dc.contributor.authorHutmacher, Dietmar W
dc.date.accessioned2018-07-22T23:08:21Z
dc.date.available2018-07-22T23:08:21Z
dc.date.issued2016
dc.identifier.issn1754-2189
dc.identifier.doi10.1038/nprot.2016.037
dc.identifier.urihttp://hdl.handle.net/10072/343838
dc.description.abstractProgress in advancing a system-level understanding of the complexity of human tissue development and regeneration is hampered by a lack of biological model systems that recapitulate key aspects of these processes in a physiological context. Hence, growing demand by cell biologists for organ-specific extracellular mimics has led to the development of a plethora of 3D cell culture assays based on natural and synthetic matrices. We developed a physiological microenvironment of semisynthetic origin, called gelatin methacryloyl (GelMA)-based hydrogels, which combine the biocompatibility of natural matrices with the reproducibility, stability and modularity of synthetic biomaterials. We describe here a step-by-step protocol for the preparation of the GelMA polymer, which takes 1–2 weeks to complete, and which can be used to prepare hydrogel-based 3D cell culture models for cancer and stem cell research, as well as for tissue engineering applications. We also describe quality control and validation procedures, including how to assess the degree of GelMA functionalization and mechanical properties, to ensure reproducibility in experimental and animal studies.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherNature Publishing Group
dc.relation.ispartofpagefrom727
dc.relation.ispartofpageto746
dc.relation.ispartofissue4
dc.relation.ispartofjournalNature Protocols
dc.relation.ispartofvolume11
dc.subject.fieldofresearchChemical sciences
dc.subject.fieldofresearchBiological sciences
dc.subject.fieldofresearchBiochemistry and cell biology not elsewhere classified
dc.subject.fieldofresearchBiomedical and clinical sciences
dc.subject.fieldofresearchcode34
dc.subject.fieldofresearchcode31
dc.subject.fieldofresearchcode310199
dc.subject.fieldofresearchcode32
dc.titleFunctionalization, preparation and use of cell-laden gelatin methacryloyl-based hydrogels as modular tissue culture platforms
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dc.description.versionAccepted Manuscript (AM)
gro.rights.copyright© 2016 Nature Publishing Group. This is the author-manuscript version of this paper. Reproduced in accordance with the copyright policy of the publisher. Please refer to the journal website for access to the definitive, published version.
gro.hasfulltextFull Text
gro.griffith.authorKaemmerer, Elke


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