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dc.contributor.authorSedaghat, Bita
dc.contributor.authorStephenson, Rachel J
dc.contributor.authorGiddam, Ashwini Kumar
dc.contributor.authorEskandari, Sharareh
dc.contributor.authorApte, Simon H
dc.contributor.authorPattinson, David J
dc.contributor.authorDoolan, Denise L
dc.contributor.authorToth, Istvan
dc.date.accessioned2017-08-11T05:21:20Z
dc.date.available2017-08-11T05:21:20Z
dc.date.issued2016
dc.identifier.issn1043-1802
dc.identifier.doi10.1021/acs.bioconjchem.5b00547
dc.identifier.urihttp://hdl.handle.net/10072/343861
dc.description.abstractPresent on the surface of antigen presenting cells (APCs), the mannose receptor (MR) has long been recognized as a front-line receptor in pathogen recognition. During the past decade many attempts have been made to target this receptor for applications including vaccine and drug development. In the present study, a library of vaccine constructs comprising fluorescently labeled mannosylated lipid-dendrimers that contained the ovalbumin CD4+ epitope, OVA323–339, as the model peptide antigen were synthesized using fluorenylmethyloxycarbonyl (Fmoc) solid phase peptide synthesis (SPPS). The vaccine constructs were designed with an alanine spacer between the O-linked mannose moieties to investigate the impact of distance between the mannose units on receptor-mediated uptake and/or binding in APCs. Uptake studies performed on F4/80+ and CD11c+ cells showed significant uptake and/or binding for lipopeptides containing mannose, and also the lipopeptide without mannose when compared to the control peptides (peptide with no lipid and peptide with no mannose and no lipid). Furthermore, mannan inhibition assays demonstrated that uptake of the mannosylated and lipidated peptides was receptor mediated. To address the specificity of receptor uptake, surface plasmon resonance studies were performed using biacore technology and confirmed high affinity of the mannosylated and lipidated vaccine constructs toward the MR. These studies confirm that both mannose and lipid moieties play significant roles in receptor-mediated uptake on APCs, potentially facilitating vaccine development.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherAmerican Chemical Society
dc.relation.ispartofpagefrom533
dc.relation.ispartofpageto548
dc.relation.ispartofissue3
dc.relation.ispartofjournalBioconjugate Chemistry
dc.relation.ispartofvolume27
dc.subject.fieldofresearchMedicinal and Biomolecular Chemistry not elsewhere classified
dc.subject.fieldofresearchMedicinal and Biomolecular Chemistry
dc.subject.fieldofresearchOrganic Chemistry
dc.subject.fieldofresearchBiochemistry and Cell Biology
dc.subject.fieldofresearchcode030499
dc.subject.fieldofresearchcode0304
dc.subject.fieldofresearchcode0305
dc.subject.fieldofresearchcode0601
dc.titleSynthesis of Mannosylated Lipopeptides with Receptor Targeting Properties
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.hasfulltextNo Full Text
gro.griffith.authorEskandari, Sharareh


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