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dc.contributor.authorYerbanga, RS
dc.contributor.authorLucantoni, L
dc.contributor.authorOuédraogo, RK
dc.contributor.authorDa, DF
dc.contributor.authorYao, FA
dc.contributor.authorYaméogo, KB
dc.contributor.authorChurcher, TS
dc.contributor.authorLupidi, G
dc.contributor.authorTaglialatela-Scafati, O
dc.contributor.authorGouagna, LC
dc.contributor.authorCohuet, A
dc.contributor.authorChristophides, GK
dc.contributor.authorOuédraogo, JB
dc.contributor.authorHabluetzel, A
dc.date.accessioned2017-08-11T05:40:16Z
dc.date.available2017-08-11T05:40:16Z
dc.date.issued2014
dc.identifier.issn1756-3305
dc.identifier.doi10.1186/1756-3305-7-185
dc.identifier.urihttp://hdl.handle.net/10072/343867
dc.description.abstractBackground: Targeting the stages of the malaria parasites responsible for transmission from the human host to the mosquito vector is a key pharmacological strategy for malaria control. Research efforts to identify compounds that are active against these stages have significantly increased in recent years. However, at present, only two drugs are available, namely primaquine and artesunate, which reportedly act on late stage gametocytes. Methods: In this study, we assessed the antiplasmodial effects of 5 extracts obtained from the neem tree Azadirachta indica and Guiera senegalensis against the early vector stages of Plasmodium falciparum, using field isolates. In an ex vivo assay gametocytaemic blood was supplemented with the plant extracts and offered to Anopheles coluzzii females by membrane feeding. Transmission blocking activity was evaluated by assessing oocyst prevalence and density on the mosquito midguts. Results: Initial screening of the 5 plant extracts at 250 ppm revealed transmission blocking activity in two neem preparations. Up to a concentration of 70 ppm the commercial extract NeemAzal® completely blocked transmission and at 60 ppm mosquitoes of 4 out of 5 replicate groups remained uninfected. Mosquitoes fed on the ethyl acetate phase of neem leaves at 250 ppm showed a reduction in oocyst prevalence of 59.0% (CI95 12.0 - 79.0; p < 10−4 ) and in oocyst density of 90.5% (CI95 86.0 - 93.5; p < 10−4 ), while the ethanol extract from the same plant part did not exhibit any activity. No evidence of transmission blocking activity was found using G. senegalensis ethyl acetate extract from stem galls. Conclusions: The results of this study highlight the potential of antimalarial plants for the discovery of novel transmission blocking molecules, and open up the potential of developing standardized transmission blocking herbal formulations as malaria control tools to complement currently used antimalarial drugs and combination treatments
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherBioMed Central
dc.relation.ispartofpagefrom185-1
dc.relation.ispartofpageto185-10
dc.relation.ispartofissue1
dc.relation.ispartofjournalParasites and Vectors
dc.relation.ispartofvolume7
dc.subject.fieldofresearchMedical Microbiology not elsewhere classified
dc.subject.fieldofresearchMedical Microbiology
dc.subject.fieldofresearchPublic Health and Health Services
dc.subject.fieldofresearchcode110899
dc.subject.fieldofresearchcode1108
dc.subject.fieldofresearchcode1117
dc.titleTransmission blocking activity of Azadirachta indica and Guiera senegalensis extracts on the sporogonic development of Plasmodium falciparum field isolates in Anopheles coluzzii mosquitoes
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttp://creativecommons.org/licenses/by/4.0/
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© 2014 Yerbanga et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
gro.hasfulltextFull Text
gro.griffith.authorLucantoni, Leonardo


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