Show simple item record

dc.contributor.authorWu, Hsing-Juen_US
dc.contributor.authorSeib, Kateen_US
dc.contributor.authorSrikhanta, Yogithaen_US
dc.contributor.authorEdwards, Jenniferen_US
dc.contributor.authorKidd, Stephen P.en_US
dc.contributor.authorL. Maguire, Tinaen_US
dc.contributor.authorHamilton, Amandaen_US
dc.contributor.authorPan, Kuan-Tinen_US
dc.contributor.authorHsiao, He-Hsuanen_US
dc.contributor.authorYao, Chen-Wenen_US
dc.contributor.authorM. Grimmond, Seanen_US
dc.contributor.authorA. Apicella, Michaelen_US
dc.contributor.authorG. McEwan, Alastairen_US
dc.contributor.authorH-J. Wang, Andrewen_US
dc.contributor.authorJennings, Michaelen_US
dc.date.accessioned2017-04-24T10:31:32Z
dc.date.available2017-04-24T10:31:32Z
dc.date.issued2010en_US
dc.date.modified2010-10-06T06:56:16Z
dc.identifier.issn1874-3919en_US
dc.identifier.doi10.1016/j.jprot.2009.12.001en_AU
dc.identifier.urihttp://hdl.handle.net/10072/34411
dc.description.abstractNeisseria gonorrhoeae has evolved a complex and novel network of oxidative stress responses, including defence mechanisms that are dependent on manganese (Mn). We performed systematic analyses at the transcriptomic and proteomic (1D SDS-PAGE and Isotope-Coded Affinity Tag [ICAT]) levels to investigate the global expression changes that take place in a high Mn environment, which results in a Mn-dependent oxidative stress resistance phenotype. These studies revealed that there were proteins regulated at the post-transcriptional level under conditions of increased Mn concentration, including proteins involved in virulence (e.g., pilin, a key adhesin), oxidative stress defence (e.g., superoxide dismutase), cellular metabolism, protein synthesis, RNA processing and cell division. Mn regulation of inorganic pyrophosphatase (Ppa) indicated the potential involvement of phosphate metabolism in the Mn-dependent oxidative stress defence. A detailed analysis of the role of Ppa and polyphosphate kinase (Ppk) in the gonococcal oxidative stress response revealed that ppk and ppa mutant strains showed increased resistance to oxidative stress. Investigation of these mutants grown with high Mn suggests that phosphate and pyrophosphate are involved in Mn-dependent oxidative stress resistance.en_US
dc.description.peerreviewedYesen_US
dc.description.publicationstatusYesen_AU
dc.languageEnglishen_US
dc.language.isoen_AU
dc.publisherElsevieren_US
dc.publisher.placeNetherlandsen_US
dc.relation.ispartofstudentpublicationNen_AU
dc.relation.ispartofpagefrom899en_US
dc.relation.ispartofpageto916en_US
dc.relation.ispartofissue5en_US
dc.relation.ispartofjournalJournal of Proteomicsen_US
dc.relation.ispartofvolume73en_US
dc.rights.retentionYen_AU
dc.subject.fieldofresearchMicrobial Geneticsen_US
dc.subject.fieldofresearchcode060503en_US
dc.titleManganese regulation of virulence factors and oxidative stress resistance in Neisseria gonorrhoeaeen_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
gro.date.issued2010
gro.hasfulltextNo Full Text


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record