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  • A novel in vitro image-based assay identifies new drug leads for giardiasis

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    Author(s)
    Hart, Christopher JS
    Munro, Taylah
    Andrews, Katherine T
    Ryan, John H
    Riches, Andrew G
    Skinner-Adams, Tina S
    Griffith University Author(s)
    Andrews, Katherine T.
    Skinner-Adams, Tina
    Hart, Christopher J.
    Munro, Taylah AM.
    Year published
    2017
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    Abstract
    Giardia duodenalis is an intestinal parasite that causes giardiasis, a widespread human gastrointestinal disease. Treatment of giardiasis relies on a small arsenal of compounds that can suffer from limitations including side-effects, variable treatment efficacy and parasite drug resistance. Thus new anti-Giardia drug leads are required. The search for new compounds with anti-Giardia activity currently depends on assays that can be labour-intensive, expensive and restricted to measuring activity at a single time-point. Here we describe a new in vitro assay to assess anti-Giardia activity. This image-based assay utilizes the ...
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    Giardia duodenalis is an intestinal parasite that causes giardiasis, a widespread human gastrointestinal disease. Treatment of giardiasis relies on a small arsenal of compounds that can suffer from limitations including side-effects, variable treatment efficacy and parasite drug resistance. Thus new anti-Giardia drug leads are required. The search for new compounds with anti-Giardia activity currently depends on assays that can be labour-intensive, expensive and restricted to measuring activity at a single time-point. Here we describe a new in vitro assay to assess anti-Giardia activity. This image-based assay utilizes the Perkin-Elmer Operetta® and permits automated assessment of parasite growth at multiple time points without cell-staining. Using this new approach, we assessed the “Malaria Box” compound set for anti-Giardia activity. Three compounds with sub-μM activity (IC50 0.6–0.9 μM) were identified as potential starting points for giardiasis drug discovery.
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    Journal Title
    International Journal for Parasitology: Drugs and Drug Resistance
    Volume
    7
    DOI
    https://doi.org/10.1016/j.ijpddr.2017.01.005
    Copyright Statement
    © 2017 The Authors. Published by Elsevier Ltd on behalf of Australian Society for Parasitology. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, providing that the work is properly cited.
    Subject
    Medical Microbiology not elsewhere classified
    Medical Microbiology
    Publication URI
    http://hdl.handle.net/10072/344537
    Collection
    • Journal articles

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