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dc.contributor.authorDrew, Roderick
dc.contributor.authorAshmore, Sarah
dc.contributor.authorAzimi-Tabrizi, Mahmoud
dc.contributor.editorM. Souza, R. Drew
dc.date.accessioned2017-05-03T12:48:14Z
dc.date.available2017-05-03T12:48:14Z
dc.date.issued2010
dc.date.modified2010-10-08T06:55:44Z
dc.identifier.issn05677572
dc.identifier.urihttp://hdl.handle.net/10072/34467
dc.description.abstractPapaya (Carica papaya) has been used in our laboratory with a range of technologies for ex situ conservation of germplasm. It represents a good model species, as it has been the subject of major research efforts in tissue culture and biotechnology. We have investigated seed storage behaviour via desiccation curves based on accurate water activity measurements and a range of storage environments from liquid nitrogen to 15ï15% RH. Treatments have been optimised to break dormancy and to ensure germination after seed storage. Twenty years of accumulated experience in papaya cell and tissue culture has facilitated our investigation of cryopreservation protocols, including vitrification based techniques, encapsulation and slow freezing, on a range of in vitro tissues from shoot tips to somatic embryos. This research has led to the development of optimum protocols for ex situ conservation of papaya germplasm, both as seed and vegetative tissue which have application to other tropical fruit species. Plants that were recovered post-cryopreservation have been screened for changes in genomic DNA and DNA methylation using the Polymerase Chain Reaction (PCR) based Randomly Amplified DNA Fingerprinting (RAF) and Amplified DNA Methylation Polymorphism (AMP) techniques, and are being evaluated in field plantings. Similarly, work on embryo culture techniques in our lab have contributed to the success of intergeneric breeding programs. Efficient molecular markers have been employed to assess genetic diversity within and between papaya genotypes and related Vasconcella species.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherInternational Society for Horticultural Science
dc.publisher.placeBelgium
dc.publisher.urihttp://www.actahort.org/
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom101
dc.relation.ispartofpageto107
dc.relation.ispartofjournalActa Horticulturae
dc.relation.ispartofvolume864
dc.rights.retentionY
dc.subject.fieldofresearchPlant Biology not elsewhere classified
dc.subject.fieldofresearchPlant Biology
dc.subject.fieldofresearchHorticultural Production
dc.subject.fieldofresearchcode060799
dc.subject.fieldofresearchcode0607
dc.subject.fieldofresearchcode0706
dc.titlePapaya as a model tropical fruit species for the development of conservation and breeding technologies
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.rights.copyright© 2010 International Society for Horticultural Science. Self-archiving of the author-manuscript version is not yet supported by this publisher. Please refer to the journal link for access to the definitive, published version or contact the author for more information.
gro.date.issued2010
gro.hasfulltextNo Full Text
gro.griffith.authorAshmore, Sarah
gro.griffith.authorAzimi-Tabrizi, Mahmoud
gro.griffith.authorDrew, Roderick A.


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