A multiplex microplatform for the detection of multiple DNA methylation events using gold-DNA affinity

We report a new multiplexed strategy for the electrochemical detection of regional DNA methylation across multiple regions. Using the sequence dependent affinity of bisulfite treated DNA towards gold surfaces, the method integrates the high sensitivity of micro-fabricated multiplex device comprising microarray of gold electrodes, with the powerful multiplexing capability of multiplex-PCR. The synergy of this combination enables the monitoring of the methylation changes across several genomic regions simultaneously from as low as 500 pg/µl of DNA with no sequencing requirement.We report a new multiplexed strategy for the electrochemical detection of regional DNA methylation across multiple regions. Using the sequence dependent affinity of bisulfite treated DNA towards gold surfaces, the method integrates the high sensitivity of micro-fabricated multiplex device comprising microarray of gold electrodes, with the powerful multiplexing capability of multiplex-PCR. The synergy of this combination enables the monitoring of the methylation changes across several genomic regions simultaneously from as low as 500 pg/µl of DNA with no sequencing requirement.
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Journal Title

Analyst

DOI

https://doi.org/10.1039/C7AN00611J

Copyright Statement

© 2017 Royal Society of Chemistry. This is the author-manuscript version of this paper. Reproduced in accordance with the copyright policy of the publisher. Please refer to the journal website for access to the definitive, published version.

Note

This publication has been entered into Griffith Research Online as an Advanced Online Version.

Subject

Analytical Chemistry not elsewhere classified

Publication URI

http://hdl.handle.net/10072/344962

Collection

Journal articles