A multiplex microplatform for the detection of multiple DNA methylation events using gold-DNA affinity

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Author(s)
Ibn Sina, Abu Ali
Foster, Matthew Thomas
Korbie, Darren
Carrascosa, Laura G
Shiddiky, Muhammad JA
Gao, Jing
Dey, Shuvashis
Trau, Matt
Griffith University Author(s)
Year published
2017
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Abstract
We report a new multiplexed strategy for the electrochemical detection of regional DNA methylation across multiple regions. Using the sequence dependent affinity of bisulfite treated DNA towards gold surfaces, the method integrates the high sensitivity of micro-fabricated multiplex device comprising microarray of gold electrodes, with the powerful multiplexing capability of multiplex-PCR. The synergy of this combination enables the monitoring of the methylation changes across several genomic regions simultaneously from as low as 500 pg/µl of DNA with no sequencing requirement.
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© 2017 Royal Society of Chemistry. This is the author-manuscript version of this paper. Reproduced in accordance with the copyright policy of the publisher. Please refer to the journal website for access to the definitive, published version.
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This publication has been entered into Griffith Research Online as an Advanced Online Version.
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Analytical Chemistry not elsewhere classified
Analytical Chemistry
Other Chemical Sciences
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