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  • A multiplex microplatform for the detection of multiple DNA methylation events using gold-DNA affinity

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    Accepted Manuscript (AM)
    Author(s)
    Ibn Sina, Abu Ali
    Foster, Matthew Thomas
    Korbie, Darren
    Carrascosa, Laura G
    Shiddiky, Muhammad JA
    Gao, Jing
    Dey, Shuvashis
    Trau, Matt
    Griffith University Author(s)
    Shiddiky, Muhammad J.
    Year published
    2017
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    Abstract
    We report a new multiplexed strategy for the electrochemical detection of regional DNA methylation across multiple regions. Using the sequence dependent affinity of bisulfite treated DNA towards gold surfaces, the method integrates the high sensitivity of micro-fabricated multiplex device comprising microarray of gold electrodes, with the powerful multiplexing capability of multiplex-PCR. The synergy of this combination enables the monitoring of the methylation changes across several genomic regions simultaneously from as low as 500 pg/µl of DNA with no sequencing requirement.We report a new multiplexed strategy for the electrochemical detection of regional DNA methylation across multiple regions. Using the sequence dependent affinity of bisulfite treated DNA towards gold surfaces, the method integrates the high sensitivity of micro-fabricated multiplex device comprising microarray of gold electrodes, with the powerful multiplexing capability of multiplex-PCR. The synergy of this combination enables the monitoring of the methylation changes across several genomic regions simultaneously from as low as 500 pg/µl of DNA with no sequencing requirement.
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    Journal Title
    Analyst
    DOI
    https://doi.org/10.1039/C7AN00611J
    Copyright Statement
    © 2017 Royal Society of Chemistry. This is the author-manuscript version of this paper. Reproduced in accordance with the copyright policy of the publisher. Please refer to the journal website for access to the definitive, published version.
    Note
    This publication has been entered into Griffith Research Online as an Advanced Online Version.
    Subject
    Analytical chemistry
    Analytical chemistry not elsewhere classified
    Other chemical sciences
    Publication URI
    http://hdl.handle.net/10072/344962
    Collection
    • Journal articles

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