dc.contributor.author | Ibn Sina, Abu Ali | |
dc.contributor.author | Foster, Matthew Thomas | |
dc.contributor.author | Korbie, Darren | |
dc.contributor.author | Carrascosa, Laura G | |
dc.contributor.author | Shiddiky, Muhammad JA | |
dc.contributor.author | Gao, Jing | |
dc.contributor.author | Dey, Shuvashis | |
dc.contributor.author | Trau, Matt | |
dc.date.accessioned | 2018-07-30T01:30:30Z | |
dc.date.available | 2018-07-30T01:30:30Z | |
dc.date.issued | 2017 | |
dc.identifier.issn | 0003-2654 | |
dc.identifier.doi | 10.1039/C7AN00611J | |
dc.identifier.uri | http://hdl.handle.net/10072/344962 | |
dc.description.abstract | We report a new multiplexed strategy for the electrochemical detection of regional DNA methylation across multiple regions. Using the sequence dependent affinity of bisulfite treated DNA towards gold surfaces, the method integrates the high sensitivity of micro-fabricated multiplex device comprising microarray of gold electrodes, with the powerful multiplexing capability of multiplex-PCR. The synergy of this combination enables the monitoring of the methylation changes across several genomic regions simultaneously from as low as 500 pg/µl of DNA with no sequencing requirement. | |
dc.description.peerreviewed | Yes | |
dc.language | English | |
dc.language.iso | eng | |
dc.publisher | Royal Society of Chemistry (RSC) Publications | |
dc.relation.ispartofpagefrom | 1 | |
dc.relation.ispartofpageto | 6 | |
dc.relation.ispartofjournal | Analyst | |
dc.subject.fieldofresearch | Analytical chemistry | |
dc.subject.fieldofresearch | Analytical chemistry not elsewhere classified | |
dc.subject.fieldofresearch | Other chemical sciences | |
dc.subject.fieldofresearchcode | 3401 | |
dc.subject.fieldofresearchcode | 340199 | |
dc.subject.fieldofresearchcode | 3499 | |
dc.title | A multiplex microplatform for the detection of multiple DNA methylation events using gold-DNA affinity | |
dc.type | Journal article | |
dc.type.description | C1 - Articles | |
dc.type.code | C - Journal Articles | |
dc.description.version | Accepted Manuscript (AM) | |
gro.description.notepublic | This publication has been entered into Griffith Research Online as an Advanced Online Version. | |
gro.rights.copyright | © 2017 Royal Society of Chemistry. This is the author-manuscript version of this paper. Reproduced in accordance with the copyright policy of the publisher. Please refer to the journal website for access to the definitive, published version. | |
gro.hasfulltext | Full Text | |
gro.griffith.author | Shiddiky, Muhammad J. | |