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dc.contributor.authorMatigian, Nicholas
dc.contributor.authorAbrahamsen, Greger
dc.contributor.authorSutharsan, Ratneswary
dc.contributor.authorCook, Anthony L
dc.contributor.authorVitale, Alejandra M
dc.contributor.authorNouwens, Amanda
dc.contributor.authorBellette, Bernadette
dc.contributor.authorAn, Jiyuan
dc.contributor.authorAnderson, Matthew
dc.contributor.authorBeckhouse, Anthony G
dc.contributor.authorBennebroek, Maikel
dc.contributor.authorCecil, Rowena
dc.contributor.authorChalk, Alistair M
dc.contributor.authorCochrane, Julie
dc.contributor.authorFan, Yongjun
dc.contributor.authorFeron, Francois
dc.contributor.authorMcCurdy, Richard
dc.contributor.authorMcGrath, John J
dc.contributor.authorMurrell, Wayne
dc.contributor.authorPerry, Chris
dc.contributor.authorRaju, Jyothy
dc.contributor.authorRavishankar, Sugandha
dc.contributor.authorSilburn, Peter A
dc.contributor.authorSutherland, Greg T
dc.contributor.authorMahler, Stephen
dc.contributor.authorMellick, George D
dc.contributor.authorWood, Stephen A
dc.contributor.authorSue, Carolyn M
dc.contributor.authorWells, Christine A
dc.contributor.authorMackay-Sim, Alan
dc.description.abstractThere is a pressing need for patient-derived cell models of brain diseases that are relevant and robust enough to produce the large quantities of cells required for molecular and functional analyses. We describe here a new cell model based on patient-derived cells from the human olfactory mucosa, the organ of smell, which regenerates throughout life from neural stem cells. Olfactory mucosa biopsies were obtained from healthy controls and patients with either schizophrenia, a neurodevelopmental psychiatric disorder, or Parkinson's disease, a neurodegenerative disease. Biopsies were dissociated and grown as neurospheres in defined medium. Neurosphere-derived cell lines were grown in serum-containing medium as adherent monolayers and stored frozen. By comparing 42 patient and control cell lines we demonstrated significant disease-specific alterations in gene expression, protein expression and cell function, including dysregulated neurodevelopmental pathways in schizophrenia and dysregulated mitochondrial function, oxidative stress and xenobiotic metabolism in Parkinson's disease. The study has identified new candidate genes and cell pathways for future investigation. Fibroblasts from schizophrenia patients did not show these differences. Olfactory neurosphere-derived cells have many advantages over embryonic stem cells and induced pluripotent stem cells as models for brain diseases. They do not require genetic reprogramming and they can be obtained from adults with complex genetic diseases. They will be useful for understanding disease aetiology, for diagnostics and for drug discovery.
dc.publisherThe Company of Biologists Ltd.
dc.publisher.placeUnited Kingdom
dc.relation.ispartofjournalDisease Models and Mechanisms
dc.subject.fieldofresearchSystems Biology
dc.subject.fieldofresearchBiological Sciences
dc.subject.fieldofresearchMedical and Health Sciences
dc.titleDisease-specific, neurosphere-derived cells as models for brain disorders
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.facultyGriffith Sciences, School of Natural Sciences
gro.rights.copyrightSelf-archiving of the author-manuscript version is not yet supported by this publisher. Please refer to the journal link for access to the definitive, published version or contact the authors for more information.
gro.hasfulltextNo Full Text
gro.griffith.authorMackay-Sim, Alan
gro.griffith.authorMurrell, Wayne G.
gro.griffith.authorSilburn, Peter A.
gro.griffith.authorMcCurdy, Richard
gro.griffith.authorMcGrath, John J.
gro.griffith.authorMatigian, Nicholas
gro.griffith.authorFeron, Francois
gro.griffith.authorAbrahamsen, Greger
gro.griffith.authorPerry, Christopher
gro.griffith.authorAnderson, Matthew J.
gro.griffith.authorMellick, George
gro.griffith.authorCochrane, Julie
gro.griffith.authorBellette, Bernadette
gro.griffith.authorWells, Christine
gro.griffith.authorRavishankar, Sugandha
gro.griffith.authorSutherland, Greg T.
gro.griffith.authorBeckhouse, Anthony G.
gro.griffith.authorSutharsan, Ratneswary
gro.griffith.authorCecil, Rowena
gro.griffith.authorChalk, Alistair M.
gro.griffith.authorWood, Stephen A.
gro.griffith.authorVitale, Alejandra M.
gro.griffith.authorAn, Jay
gro.griffith.authorCook, Anthony
gro.griffith.authorFan, Yongjun
gro.griffith.authorRaju, Jyothy
gro.griffith.authorBennebroek, Maikel JA.

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