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  • In vivo oxidative protein folding can be facilitated by oxidation-reduction cycling

    Author(s)
    Shouldice, Stephen R
    Cho, Seung-Hyun
    Boyd, Dana
    Heras, Begona
    Eser, Markus
    Beckwith, Jon
    Riggs, Paul
    Martin, Jennifer L
    Berkmen, Mehmet
    Griffith University Author(s)
    Martin, Jennifer
    Year published
    2010
    Metadata
    Show full item record
    Abstract
    Current dogma dictates that bacterial proteins with misoxidized disulfide bonds are shuffled into correctly oxidized states by DsbC. There are two proposed mechanisms for DsbC activity. The first involves a DsbC-only model of substrate disulfide rearrangement. The second invokes cycles of reduction and oxidation of substrate disulfide bonds by DsbC and DsbA respectively. Here, we addressed whether the second mechanism is important in vivo by identifying whether a periplasmic reductase could complement DsbC. We screened for naturally occurring periplasmic reductases in Bacteroides fragilis, a bacterium chosen because we ...
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    Current dogma dictates that bacterial proteins with misoxidized disulfide bonds are shuffled into correctly oxidized states by DsbC. There are two proposed mechanisms for DsbC activity. The first involves a DsbC-only model of substrate disulfide rearrangement. The second invokes cycles of reduction and oxidation of substrate disulfide bonds by DsbC and DsbA respectively. Here, we addressed whether the second mechanism is important in vivo by identifying whether a periplasmic reductase could complement DsbC. We screened for naturally occurring periplasmic reductases in Bacteroides fragilis, a bacterium chosen because we predicted it encodes reductases and has a reducing periplasm. We found that the B. fragilis periplasmic protein TrxP has a thioredoxin fold with an extended N-terminal region; that it is a very active reductase but a poor isomerase; and that it fully complements dsbC. These results provide direct in vivo evidence that correctly folded protein is achievable via cycles of oxidation and reduction.
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    Journal Title
    Molecular Microbiology
    Volume
    75
    Issue
    1
    DOI
    https://doi.org/10.1111/j.1365-2958.2009.06952.x
    Subject
    Biological sciences
    Biochemistry and cell biology not elsewhere classified
    Agricultural, veterinary and food sciences
    Biomedical and clinical sciences
    Publication URI
    http://hdl.handle.net/10072/345723
    Collection
    • Journal articles

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