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dc.contributor.authorWooh, JW
dc.contributor.authorKidd, RD
dc.contributor.authorMartin, JL
dc.contributor.authorKobe, B
dc.date.accessioned2017-09-01T04:03:51Z
dc.date.available2017-09-01T04:03:51Z
dc.date.issued2003
dc.identifier.issn2059-7983
dc.identifier.doi10.1107/S0907444903002919
dc.identifier.urihttp://hdl.handle.net/10072/345725
dc.description.abstractSparse-matrix sampling using commercially available crystallization screen kits has become the most popular way of determining the preliminary crystallization conditions for macromolecules. In this study, the efficiency of three commercial screening kits, Crystal Screen and Crystal Screen 2 (Hampton Research), Wizard Screens I and II (Emerald BioStructures) and Personal Structure Screens 1 and 2 (Molecular Dimensions), has been compared using a set of 19 diverse proteins. 18 proteins yielded crystals using at least one crystallization screen. Surprisingly, Crystal Screens and Personal Structure Screens showed dramatically different results, although most of the crystallization formulations are identical as listed by the manufacturers. Higher molecular weight polyethylene glycols and mixed precipitants were found to be the most effective precipitants in this study.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherWiley-Blackwell Publishing
dc.relation.ispartofpagefrom769
dc.relation.ispartofpageto772
dc.relation.ispartofissue4
dc.relation.ispartofjournalActa Crystallographica Section D: Biological Crystallography
dc.relation.ispartofvolumeD59
dc.subject.fieldofresearchAnalytical chemistry not elsewhere classified
dc.subject.fieldofresearchcode340199
dc.titleComparison of three commercial sparse-matrix crystallization screens
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.hasfulltextNo Full Text
gro.griffith.authorMartin, Jennifer


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