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dc.contributor.authorIslam, Md Nazmul
dc.contributor.authorGopalan, Vinod
dc.contributor.authorHague, Md Hakimul
dc.contributor.authorMasud, Mostafa Kamal
dc.contributor.authorAl Hossain, Shahriar
dc.contributor.authorYamauchi, Yusuke
dc.contributor.authorNam-Trung, Nguyen
dc.contributor.authorLam, Alfred King-Yin
dc.contributor.authorShiddiky, Muhammad JA
dc.date.accessioned2017-09-15T06:11:32Z
dc.date.available2017-09-15T06:11:32Z
dc.date.issued2017
dc.identifier.issn0956-5663
dc.identifier.doi10.1016/j.bios.2017.06.051
dc.identifier.urihttp://hdl.handle.net/10072/346776
dc.description.abstractDespite having reliable and excellent diagnostic performances, the currently available messenger RNA (mRNA) detection methods mostly use enzymatic amplification steps of the target mRNA which is generally affected by the sample manipulations, amplification bias and longer assay time. This paper reports an amplification-free electrochemical approach for the sensitive and selective detection of mRNA using a screen-printed gold electrode (SPE-Au). The target mRNA is selectively isolated by magnetic separation and adsorbed directly onto an unmodified SPE-Au. The surface-attached mRNA is then measured by differential pulse voltammetry (DPV) in the presence of [Fe(CN)6]4-/3- redox system. This method circumvents the PCR amplification steps as well as simplifies the assay construction by avoiding multiple steps involved in conventional biosensing approaches of using recognition and transduction layers. Our method has demonstrated good sensitivity (LOD = 1.0 pM) and reproducibility (% RSD = <5%, for n = 3) for detecting FAM134B mRNA in two cancer cell lines and a small cohort of clinical samples (number of samples = 26) collected from patients with oesophageal cancer. The analytical performance of our method is validated with a standard qRT-PCR analysis. We believe that our PCR-free approach holds a great promise for the analysis of tumor-specific mRNA in clinical samples.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier
dc.relation.ispartofpagefrom227
dc.relation.ispartofpageto233
dc.relation.ispartofjournalBiosensors and Bioelectronics
dc.relation.ispartofvolume98
dc.subject.fieldofresearchAnalytical chemistry
dc.subject.fieldofresearchElectrochemistry
dc.subject.fieldofresearchBiomedical engineering
dc.subject.fieldofresearchNanotechnology
dc.subject.fieldofresearchcode3401
dc.subject.fieldofresearchcode340604
dc.subject.fieldofresearchcode4003
dc.subject.fieldofresearchcode4018
dc.titleA PCR-free electrochemical method for messenger RNA detection in cancer tissue samples
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.description.versionAccepted Manuscript (AM)
gro.facultyGriffith Sciences, School of Natural Sciences
gro.rights.copyright© 2017 Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (http://creativecommons.org/licenses/by-nc-nd/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, providing that the work is properly cited.
gro.hasfulltextFull Text
gro.griffith.authorLam, Alfred K.
gro.griffith.authorGopalan, Vinod
gro.griffith.authorNguyen, Nam-Trung
gro.griffith.authorShiddiky, Muhammad J.


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