Show simple item record

dc.contributor.authorKing, Gordon J
dc.contributor.authorChen, Kai-En
dc.contributor.authorRobin, Gautier
dc.contributor.authorForwood, Jade K
dc.contributor.authorHeras, Begona
dc.contributor.authorThakur, Anil S
dc.contributor.authorKobe, Bostjan
dc.contributor.authorBlomberg, Simon P
dc.contributor.authorMartin, Jennifer L
dc.date.accessioned2017-09-22T04:26:30Z
dc.date.available2017-09-22T04:26:30Z
dc.date.issued2009
dc.identifier.issn1932-6203
dc.identifier.doi10.1371/journal.pone.0007851
dc.identifier.urihttp://hdl.handle.net/10072/347154
dc.description.abstractBackground: Protein crystallisation screening involves the parallel testing of large numbers of candidate conditions with the aim of identifying conditions suitable as a starting point for the production of diffraction quality crystals. Generally, condition screening is performed in 96-well plates. While previous studies have examined the effects of protein construct, protein purity, or crystallisation condition ingredients on protein crystallisation, few have examined the effect of the crystallisation plate. Methodology/Principal Findings: We performed a statistically rigorous examination of protein crystallisation, and evaluated interactions between crystallisation success and plate row/column, different plates of same make, different plate makes and different proteins. From our analysis of protein crystallisation, we found a significant interaction between plate make and the specific protein being crystallised. Conclusions/Significance: Protein crystal structure determination is the principal method for determining protein structure but is limited by the need to produce crystals of the protein under study. Many important proteins are difficult to crystallise, so that identification of factors that assist crystallisation could open up the structure determination of these more challenging targets. Our findings suggest that protein crystallisation success may be improved by matching a protein with its optimal plate make.
dc.description.peerreviewedYes
dc.languageEnglish
dc.publisherPublic Library of Sciences
dc.relation.ispartofpagefrome7851-1
dc.relation.ispartofpagetoe7851-5
dc.relation.ispartofissue11
dc.relation.ispartofjournalPLoS One
dc.relation.ispartofvolume4
dc.subject.fieldofresearchBiochemistry and Cell Biology not elsewhere classified
dc.subject.fieldofresearchcode060199
dc.titleInteraction between plate make and protein in protein crystallisation screening
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttp://creativecommons.org/licenses/by/4.0/
dc.description.versionPublished
gro.rights.copyright© 2009 King et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
gro.hasfulltextFull Text
gro.griffith.authorMartin, Jennifer
gro.griffith.authorKing, Gordon


Files in this item

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record