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dc.contributor.authorKing, Gordon J.
dc.contributor.authorChen, Kai-En
dc.contributor.authorRobin, Gautier
dc.contributor.authorForwood, Jade K.
dc.contributor.authorHeras, Begona
dc.contributor.authorThakur, Anil S.
dc.contributor.authorKobe, Bostjan
dc.contributor.authorBlomberg, Simon P.
dc.contributor.authorMartin, Jennifer L.
dc.date.accessioned2017-09-22T04:26:30Z
dc.date.available2017-09-22T04:26:30Z
dc.date.issued2009
dc.identifier.issn1932-6203en_US
dc.identifier.doi10.1371/journal.pone.0007851en_US
dc.identifier.urihttp://hdl.handle.net/10072/347154
dc.description.abstractBackground: Protein crystallisation screening involves the parallel testing of large numbers of candidate conditions with the aim of identifying conditions suitable as a starting point for the production of diffraction quality crystals. Generally, condition screening is performed in 96-well plates. While previous studies have examined the effects of protein construct, protein purity, or crystallisation condition ingredients on protein crystallisation, few have examined the effect of the crystallisation plate. Methodology/Principal Findings: We performed a statistically rigorous examination of protein crystallisation, and evaluated interactions between crystallisation success and plate row/column, different plates of same make, different plate makes and different proteins. From our analysis of protein crystallisation, we found a significant interaction between plate make and the specific protein being crystallised. Conclusions/Significance: Protein crystal structure determination is the principal method for determining protein structure but is limited by the need to produce crystals of the protein under study. Many important proteins are difficult to crystallise, so that identification of factors that assist crystallisation could open up the structure determination of these more challenging targets. Our findings suggest that protein crystallisation success may be improved by matching a protein with its optimal plate make.en_US
dc.description.peerreviewedYesen_US
dc.languageEnglishen_US
dc.publisherPublic Library of Sciencesen_US
dc.relation.ispartofpagefrome7851-1en_US
dc.relation.ispartofpagetoe7851-5en_US
dc.relation.ispartofissue11en_US
dc.relation.ispartofjournalPLoS Oneen_US
dc.relation.ispartofvolume4en_US
dc.subject.fieldofresearchBiochemistry and Cell Biology not elsewhere classifieden_US
dc.subject.fieldofresearchcode060199en_US
dc.titleInteraction between plate make and protein in protein crystallisation screeningen_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
dcterms.licensehttp://creativecommons.org/licenses/by/4.0/en_US
dc.description.versionPublisheden_US
gro.rights.copyright© 2009 King et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
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