• myGriffith
    • Staff portal
    • Contact Us⌄
      • Future student enquiries 1800 677 728
      • Current student enquiries 1800 154 055
      • International enquiries +61 7 3735 6425
      • General enquiries 07 3735 7111
      • Online enquiries
      • Staff phonebook
    View Item 
    •   Home
    • Griffith Research Online
    • Journal articles
    • View Item
    • Home
    • Griffith Research Online
    • Journal articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

  • All of Griffith Research Online
    • Communities & Collections
    • Authors
    • By Issue Date
    • Titles
  • This Collection
    • Authors
    • By Issue Date
    • Titles
  • Statistics

  • Most Popular Items
  • Statistics by Country
  • Most Popular Authors
  • Support

  • Contact us
  • FAQs
  • Admin login

  • Login
  • Gold-Loaded Nanoporous Ferric Oxide Nanocubes with Peroxidase-Mimicking Activity for Electrocatalytic and Colorimetric Detection of Autoantibody

    Thumbnail
    View/Open
    MasudPUB4924.pdf (1.236Mb)
    File version
    Accepted Manuscript (AM)
    Author(s)
    Masud, Mostafa Kamal
    Yadav, Sharda
    Isam, Md Nazmul
    Nam-Trung, Nguyen
    Salomon, Carlos
    Kline, Richard
    Alamri, Hatem R
    Alothman, Zeid A
    Yamauchi, Yusuke
    Hossain, Md Shahriar A
    Shiddiky, Muhammad JA
    Griffith University Author(s)
    Nguyen, Nam-Trung
    Shiddiky, Muhammad J.
    Yadav, Sharda
    Masud, Mostafa Kamal K.
    Islam, Nazmul
    Year published
    2017
    Metadata
    Show full item record
    Abstract
    Enzyme-mimicking activity of iron oxide based nanostructures have provided a significant advantage in developing advanced molecular sensors for biomedical and environmental applications. Herein, we introduce the horseradish peroxidase (HRP)-like activity of gold loaded nanoporous ferric oxide nanocubes (Au-NPFe2O3NC) for the development of a molecular sensor with enhanced electrocatalytic and colorimetric (naked-eye) detection of autoantibodies. The results showed that Au-NPFe2O3NC exhibits enhanced peroxidase-like activity towards the catalytic oxidation of 3,3ˊ,5,5ˊ-tertamethylbenzidine (TMB) in the presence of H2O2 at ...
    View more >
    Enzyme-mimicking activity of iron oxide based nanostructures have provided a significant advantage in developing advanced molecular sensors for biomedical and environmental applications. Herein, we introduce the horseradish peroxidase (HRP)-like activity of gold loaded nanoporous ferric oxide nanocubes (Au-NPFe2O3NC) for the development of a molecular sensor with enhanced electrocatalytic and colorimetric (naked-eye) detection of autoantibodies. The results showed that Au-NPFe2O3NC exhibits enhanced peroxidase-like activity towards the catalytic oxidation of 3,3ˊ,5,5ˊ-tertamethylbenzidine (TMB) in the presence of H2O2 at room temperature (25°C) and follows the typical Michaelis–Menten kinetics. The autoantibody sensor based on this intrinsic property of Au-NPFe2O3NC resulted excellent detection sensitivity (LOD = 0.08 U/mL) and reproducibility (% RSD = < 5% for n = 3) for analysing p53-specific autoantibodies using electrochemical and colorimetric (naked-eye) readouts. The clinical applicability of the sensor has been tested in detecting p53-specific autoantibody in plasma obtained from patients with epithelial ovarian cancer high-grade serous subtype (EOCHGS, number of samples = 2) and controls (benign, number of samples = 2). As Au-NPFe2O3NC possess high peroxidase-like activity for the oxidation of TMB in the presence of H2O2 (TMB is a common chromogenic substrate for HRP in ELISAs), we envisage that our assay could find a wide range of application in developing ELISA based sensing approaches in the field of medicine (i.e. detection of other biomarkers same as p53 autoantibody), biotechnology and environmental sciences.
    View less >
    Journal Title
    Analytical Chemistry
    Volume
    89
    DOI
    https://doi.org/10.1021/acs.analchem.7b02880
    Copyright Statement
    This document is the Accepted Manuscript version of a Published Work that appeared in final form in Analytical Chemistry, copyright 2017 American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see 10.1021/acs.analchem.7b02880.
    Note
    This publication has been entered into Griffith Research Online as an Advanced Online Version.
    Subject
    Analytical chemistry
    Analytical chemistry not elsewhere classified
    Other chemical sciences
    Publication URI
    http://hdl.handle.net/10072/347450
    Collection
    • Journal articles

    Footer

    Disclaimer

    • Privacy policy
    • Copyright matters
    • CRICOS Provider - 00233E

    Tagline

    • Gold Coast
    • Logan
    • Brisbane - Queensland, Australia
    First Peoples of Australia
    • Aboriginal
    • Torres Strait Islander