• myGriffith
    • Staff portal
    • Contact Us⌄
      • Future student enquiries 1800 677 728
      • Current student enquiries 1800 154 055
      • International enquiries +61 7 3735 6425
      • General enquiries 07 3735 7111
      • Online enquiries
      • Staff phonebook
    View Item 
    •   Home
    • Griffith Research Online
    • Journal articles
    • View Item
    • Home
    • Griffith Research Online
    • Journal articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

  • All of Griffith Research Online
    • Communities & Collections
    • Authors
    • By Issue Date
    • Titles
  • This Collection
    • Authors
    • By Issue Date
    • Titles
  • Statistics

  • Most Popular Items
  • Statistics by Country
  • Most Popular Authors
  • Support

  • Contact us
  • FAQs
  • Admin login

  • Login
  • An In Vitro Assay to Evaluate the Immunomodulatory Effects of Unrestricted Somatic Stem Cells

    Thumbnail
    View/Open
    63519_1.pdf (2.146Mb)
    Author(s)
    Ebrahimi, Hasan
    Soleimani, Masoud
    Arababadi, Mohammad Kazemi
    Ahmadbeigi, Naser
    Hassanshahi, Gholamhossein
    Farjadfar, Akbar
    Kennedy, Derek
    Griffith University Author(s)
    Kennedy, Derek D.
    Year published
    2010
    Metadata
    Show full item record
    Abstract
    Background: Unrestricted somatic stem cells (USSC) are a cord blood stem cell that have been considered as a candidate for the regulation of immune responses. Therefore, potential exists for their use in the suppression of immune response after transplantation surgery. The aim of this study was evaluation of the effect of USSC on mixed lymphocyte reaction (MLR) as a model for graft rejection. Material and methods: USSC and Mesanchymal stem cells (MSC) were isolated and cultured from cord blood and bone morrow, respectively. The immunophenotypes of USSC and MSC were evaluated by flow cytometery and titrated Quantities ...
    View more >
    Background: Unrestricted somatic stem cells (USSC) are a cord blood stem cell that have been considered as a candidate for the regulation of immune responses. Therefore, potential exists for their use in the suppression of immune response after transplantation surgery. The aim of this study was evaluation of the effect of USSC on mixed lymphocyte reaction (MLR) as a model for graft rejection. Material and methods: USSC and Mesanchymal stem cells (MSC) were isolated and cultured from cord blood and bone morrow, respectively. The immunophenotypes of USSC and MSC were evaluated by flow cytometery and titrated Quantities of USSC and MSC were co-cultured with peripheral blood lymphocytes (PBL) in an MLR to evaluate the immunomodulatory effect of these cells as a percentage of the control response. Measurements of the MLR were made using a micro ߭liquid scintillation counter Results: Results of the current study demonstrated that proliferation of lymphocytes in the MLR were decreased after treatment with USSC, in a similar fashion to that seen with MSC. Discussion: It can be concluded that USSC have similar regulatory affects as MSC on the MLR, which can be used as an indicator for potential organ rejection after transplantation. Therefore, the immunregulatory effect of these cells could be used in the clinic during organ transplantation and in the management of autoimmunity.
    View less >
    Journal Title
    Iranian Journal of Immunology
    Volume
    7
    Issue
    1
    Publisher URI
    http://iji.sums.ac.ir/
    Copyright Statement
    © 2010 Shiraz Institute for Cancer Research. This is the author-manuscript version of this paper. Reproduced in accordance with the copyright policy of the publisher. Please refer to the journal website for access to the definitive, published version.
    Subject
    Medical genetics (excl. cancer genetics)
    Publication URI
    http://hdl.handle.net/10072/34903
    Collection
    • Journal articles

    Footer

    Disclaimer

    • Privacy policy
    • Copyright matters
    • CRICOS Provider - 00233E
    • TEQSA: PRV12076

    Tagline

    • Gold Coast
    • Logan
    • Brisbane - Queensland, Australia
    First Peoples of Australia
    • Aboriginal
    • Torres Strait Islander