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  • MRI changes and complement activation correlate with epileptogenicity in a mouse model of temporal lobe epilepsy

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    KharatishviliPUB4072.pdf (3.162Mb)
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    Accepted Manuscript (AM)
    Author(s)
    Kharatishvili, Irina
    Shan, Zuyao Y
    She, David T
    Foong, Samuel
    Kurniawan, Nyoman D
    Reutens, David C
    Griffith University Author(s)
    Shan, Zack
    Year published
    2014
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    Abstract
    The complex pathogenesis of temporal lobe epilepsy includes neuronal and glial pathology, synaptic reorganization, and an immune response. However, the spatio-temporal pattern of structural changes in the brain that provide a substrate for seizure generation and modulate the seizure phenotype is yet to be completely elucidated. We used quantitative magnetic resonance imaging (MRI) to study structural changes triggered by status epilepticus (SE) and their association with epileptogenesis and with activation of complement component 3 (C3). SE was induced by injection of pilocarpine in CD1 mice. Quantitative diffusion-weighted ...
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    The complex pathogenesis of temporal lobe epilepsy includes neuronal and glial pathology, synaptic reorganization, and an immune response. However, the spatio-temporal pattern of structural changes in the brain that provide a substrate for seizure generation and modulate the seizure phenotype is yet to be completely elucidated. We used quantitative magnetic resonance imaging (MRI) to study structural changes triggered by status epilepticus (SE) and their association with epileptogenesis and with activation of complement component 3 (C3). SE was induced by injection of pilocarpine in CD1 mice. Quantitative diffusion-weighted imaging and T2 relaxometry was performed using a 16.4-Tesla MRI scanner at 3 h and 1, 2, 7, 14, 28, 35, and 49 days post-SE. Following longitudinal MRI examinations, spontaneous recurrent seizures and interictal spikes were quantified using continuous video-EEG monitoring. Immunohistochemical analysis of C3 expression was performed at 48 h, 7 days, and 4 months post-SE. MRI changes were dynamic, reflecting different outcomes in relation to the development of epilepsy. Apparent diffusion coefficient changes in the hippocampus at 7 days post-SE correlated with the severity of the evolving epilepsy. C3 activation was found in all stages of epileptogenesis within the areas with significant MRI changes and correlated with the severity of epileptic condition.
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    Journal Title
    Brain Structure and Function
    Volume
    219
    Issue
    2
    DOI
    https://doi.org/10.1007/s00429-013-0528-4
    Copyright Statement
    © 2014 pringer-Verlag Berlin Heidelberg. This is an electronic version of an article published in Brain Structure and Function, Volume 219, Issue 2, pp 683–706, 2014. Brain Structure and Function is available online at: http://link.springer.com/ with the open URL of your article.
    Subject
    Neurosciences
    Neurology and neuromuscular diseases
    Medical physiology
    Cognitive and computational psychology
    Publication URI
    http://hdl.handle.net/10072/351682
    Collection
    • Journal articles

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