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  • Phylogenomics of the reproductive parasite Wolbachia pipientis wMel: a streamlined genome overrun by mobile genetic elements

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    Author(s)
    Wu, Martin
    V. Sun, Ling
    Vamathevan, Jessica
    Riegler, Markus
    Deboy, Robert
    Brownlie, Jeremy C.
    A. McGraw, Elizaeth
    Martin, William
    Esser, Christian
    Ahmadinejad, Nahal
    Wiegand, Christian
    Madupu, Ramana
    Beanan, Maureen
    M. Brinkac, Lauren
    C. Daugherty, Sean
    Scott Durkin, A.
    F. Kolonay, James
    C. Nelson, William
    Mohamoud, Yasmin
    Lee, Perris
    Berry, Kristi
    Brook Young, M.
    Utterback, Teresa
    Weidman, Janice
    C. Nierman, William
    T. Paulsen, Ian
    E. Nelson, Karen
    Tettelin, Herve
    L. O'Neil, Scott
    A. Eisen, Jonathan
    Griffith University Author(s)
    Brownlie, Jeremy
    Year published
    2004
    Metadata
    Show full item record
    Abstract
    The complete sequence of the 1,267,782 bp genome of Wolbachia pipientis wMel, an obligate intracellular bacteria of Drosophila melanogaster, has been determined. Wolbachia, which are found in a variety of invertebrate species, are of great interest due to their diverse interactions with different hosts, which range from many forms of reproductive parasitism to mutualistic symbioses. Analysis of the wMel genome, in particular phylogenomic comparisons with other intracellular bacteria, has revealed many insights into the biology and evolution of wMel and Wolbachia in general. For example, the wMel genome is unique among ...
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    The complete sequence of the 1,267,782 bp genome of Wolbachia pipientis wMel, an obligate intracellular bacteria of Drosophila melanogaster, has been determined. Wolbachia, which are found in a variety of invertebrate species, are of great interest due to their diverse interactions with different hosts, which range from many forms of reproductive parasitism to mutualistic symbioses. Analysis of the wMel genome, in particular phylogenomic comparisons with other intracellular bacteria, has revealed many insights into the biology and evolution of wMel and Wolbachia in general. For example, the wMel genome is unique among sequenced obligate intracellular species in both being highly streamlined and containing very high levels of repetitive DNA and mobile DNA elements. This observation, coupled with multiple evolutionary reconstructions, suggests that natural selection is somewhat inefficient in wMel, most likely owing to the occurrence of repeated population bottlenecks. Genome analysis predicts many metabolic differences with the closely related Rickettsia species, including the presence of intact glycolysis and purine synthesis, which may compensate for an inability to obtain ATP directly from its host, as Rickettsia can. Other discoveries include the apparent inability of wMel to synthesize lipopolysaccharide and the presence of the most genes encoding proteins with ankyrin repeat domains of any prokaryotic genome yet sequenced. Despite the ability of wMel to infect the germline of its host, we find no evidence for either recent lateral gene transfer between wMel and D. melanogaster or older transfers between Wolbachia and any host. Evolutionary analysis further supports the hypothesis that mitochondria share a common ancestor with the a-Proteobacteria, but shows little support for the grouping of mitochondria with species in the order Rickettsiales. With the availability of the complete genomes of both species and excellent genetic tools for the host, the wMel-D. melanogaster symbiosis is now an ideal system for studying the biology and evolution of Wolbachia infections.
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    Journal Title
    PLoS Biology
    Volume
    2
    Issue
    3
    DOI
    https://doi.org/10.1371/journal.pbio.0020069
    Copyright Statement
    © 2004 Brownlie et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution License CCAL. (http://www.plos.org/journals/license.html)
    Subject
    Biological sciences
    Microbiology not elsewhere classified
    Agricultural, veterinary and food sciences
    Biomedical and clinical sciences
    Publication URI
    http://hdl.handle.net/10072/35242
    Collection
    • Journal articles

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