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dc.contributor.authorHamlet, SM
dc.contributor.editorSeymour, Gregory J.; Cullinan, Mary P.; Heng, Nicholas C.K.
dc.date.accessioned2017-10-17T12:30:17Z
dc.date.available2017-10-17T12:30:17Z
dc.date.issued2010
dc.date.modified2011-05-12T07:02:05Z
dc.identifier.isbn9781607618195
dc.identifier.doi10.1007/978-1-60761-820-1_9
dc.identifier.urihttp://hdl.handle.net/10072/35387
dc.description.abstractThe development of analytical methods enabling the accurate identification and enumeration of bacterial species colonizing the oral cavity has led to the identification of a small number of bacterial pathogens that are major factors in the etiology of periodontal disease. Further, these methods also underpin more recent epidemiological analyses of the impact of periodontal disease on general health. Given the complex milieu of over 700 species of microorganisms known to exist within the complex biofilms found in the oral cavity, the identification and enumeration of oral periodontopathogens has not been an easy task. In recent years however, some of the intrinsic limitations of the more traditional microbiological analyses previously used have been overcome with the advent of immunological and molecular analytical methods. Of the plethora of methodologies reported in the literature, the enzyme-linked immunosorbent assay (ELISA), which combines the specificity of antibody with the sensitivity of simple enzyme assays and the polymerase chain reaction (PCR), has been widely utilized in both laboratory and clinical applications. Although conventional PCR does not allow quantitation of the target organism, real-time PCR (rtPCR) has the ability to detect amplicons as they accumulate in "real time" allowing subsequent quantitation. These methods enable the accurate quantitation of as few as 102 (using rtPCR) to 104 (using ELISA) periodontopathogens in dental plaque samples.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherSpringer
dc.publisher.placeUnited Kingdom
dc.publisher.urihttps://link.springer.com/book/10.1007/978-1-60761-820-1
dc.relation.ispartofbooktitleOral Biology: Molecular Techniques and Applications
dc.relation.ispartofchapter9
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom125
dc.relation.ispartofpageto140
dc.relation.ispartofvolume666
dc.rights.retentionY
dc.subject.fieldofresearchOther chemical sciences
dc.subject.fieldofresearchBiochemistry and cell biology
dc.subject.fieldofresearchDentistry not elsewhere classified
dc.subject.fieldofresearchMedicinal and biomolecular chemistry
dc.subject.fieldofresearchcode3499
dc.subject.fieldofresearchcode3101
dc.subject.fieldofresearchcode320399
dc.subject.fieldofresearchcode3404
dc.titleQuantitative Analysis of Periodontal Pathogens by ELISA and Real-Time Polymerase Chain Reaction
dc.typeBook chapter
dc.type.descriptionB1 - Chapters
dc.type.codeB - Book Chapters
gro.facultyGriffith Health, School of Dentistry and Oral Health
gro.date.issued2010
gro.hasfulltextNo Full Text
gro.griffith.authorHamlet, Stephen


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