Poly I:C Delivery into J774.1 & RAW264.7 Macrophages Induces Rapid Cell Death
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Background: Cytosolic double-stranded RNA (dsR NA) is an important ‘molecular signature’ for the detection of intracellular viral infect ions. Although intracellular dsRNA is a known potent inducer of apoptosis , the optimal time and dose for the onset of dsRNA-mediated apoptosis ha ve not been studied in detail. Objective : To perform an accurate temporal assessment of the cell death responses in dsRNA-dependent cytotoxicity. Methods : Poly I:C (PIC), a synthetic dsRNA molecule was delivered intracellularly into J774.1 and RAW264.7 muri ne macrophages via electroporation. Cell viability was measured using the MTT assay and apoptosis was determined by sub- G0/G1 DNA content using flow cytometry. Results : Loss of cell viability was seen as early as 3h post-electroporat ion of macrophages. A signif icant increase in the sub- G0/G1 DNA content consistent with apopt osis was observed in PIC-electroporated macrophages as early as 3h post electroporation. Conclusion : Intracellular PIC delivery induces rapid macrophage cell death.
Iranian Journal of Immunology
Immunology not elsewhere classified