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dc.contributor.authorMoghadas, Hajar
dc.contributor.authorSaidi, Mohammad Said
dc.contributor.authorKashaninejad, Navid
dc.contributor.authorKiyoumarsioskouei, Amir
dc.contributor.authorNam-Trung, Nguyen
dc.date.accessioned2017-11-30T01:15:07Z
dc.date.available2017-11-30T01:15:07Z
dc.date.issued2017
dc.identifier.issn1387-2176
dc.identifier.doi10.1007/s10544-017-0215-y
dc.identifier.urihttp://hdl.handle.net/10072/354156
dc.description.abstractThis paper reports the fabrication of electrospun polydimethylsiloxane (PDMS) membranes/scaffolds that are suitable for three-dimensional (3D) cell culture. Through modification the ratio between PDMS and polymethylmethacrylate (PMMA) as carrier polymer, we report the possibility of increasing PDMS weight ratio of up to 6 for electrospinning. Increasing the PDMS content increases the fiber diameter, the pore size, and the hydrophobicity. To our best knowledge, this is the first report describing beads-free, durable and portable electrospun membrane with maximum content of PDMS suitable for cell culture applications. To show the proof-of-concept, we successfully cultured epithelial lung cancer cells on these membranes in a static well plate without surface modification. Surprisingly, due to three-dimensional (3D) and hydrophobic nature of the electrospun fibers, cells aggregated into 3D multicellular spheroids. These easily detachable and cost-effective scaffolds with controllable thicknesses and high tensile strength are good candidates for cell-stretching devices, organ-on-a-chip devices, tissue engineering and studies of non-adherent mammalian cancer stem cells.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofpagefrom74-1
dc.relation.ispartofpageto74-9
dc.relation.ispartofissue4
dc.relation.ispartofjournalBiomedical Microdevices
dc.relation.ispartofvolume19
dc.subject.fieldofresearchBiomedical Engineering not elsewhere classified
dc.subject.fieldofresearchBiomedical Engineering
dc.subject.fieldofresearchMaterials Engineering
dc.subject.fieldofresearchcode090399
dc.subject.fieldofresearchcode0903
dc.subject.fieldofresearchcode0912
dc.titleFabrication and characterization of low-cost, bead-free, durable and hydrophobic electrospun membrane for 3D cell culture
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dc.description.versionAccepted Manuscript (AM)
gro.rights.copyright© 2017 Springer US. This is an electronic version of an article published in Biomedical Microdevices, 19(74), 2017.Biomedical Microdevices is available online at: http://link.springer.com/ with the open URL of your article.
gro.hasfulltextFull Text
gro.griffith.authorNguyen, Nam-Trung
gro.griffith.authorKashaninejad, Navid


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