Increase in ammonia-oxidizing microbe abundance during degradation of alpine meadows may lead to greater soil nitrogen loss
Author(s)
Che, Rongxiao
Wang, Fang
Wang, Weijin
Zhang, Jing
Zhao, Xing
Rui, Yichao
Xu, Zhihong
Wang, Yanfen
Hao, Yanbin
Cui, Xiaoyong
Griffith University Author(s)
Year published
2017
Metadata
Show full item recordAbstract
Alpine meadows on the Tibetan Plateau have experienced severe degradation in recent decades. Although the effects of alpine meadow degradation on soil properties have been well documented, there is still a paucity of knowledge regarding the responses of nitrogen-cycling microbes (NCMs) to degradation and their links to the changes in soil properties. Here, we systematically determined the effects of degraded patch formation on soil properties (i.e., total carbon, total nitrogen, ammonium nitrogen, nitrate nitrogen, available phosphorus, dissolved organic carbon, moisture, δ15N, δ13C, and pH) and NCMs (based on nifH, amoA, ...
View more >Alpine meadows on the Tibetan Plateau have experienced severe degradation in recent decades. Although the effects of alpine meadow degradation on soil properties have been well documented, there is still a paucity of knowledge regarding the responses of nitrogen-cycling microbes (NCMs) to degradation and their links to the changes in soil properties. Here, we systematically determined the effects of degraded patch formation on soil properties (i.e., total carbon, total nitrogen, ammonium nitrogen, nitrate nitrogen, available phosphorus, dissolved organic carbon, moisture, δ15N, δ13C, and pH) and NCMs (based on nifH, amoA, narG, nirK, and nirS genes and their transcripts) across three Tibetan alpine meadows at different degradation stages. Results showed that compared to the original grassed patches, the contents of most soil nutrients (e.g., carbon, nitrogen, and phosphorus) were significantly decreased in the degraded patches across the study sites. Degraded patches also tended to have higher soil δ15N values and nitrate contents. Among the aforementioned NCMs, soil diazotrophs and denitrifiers only showed weak responses to the patch formation, while ammonia-oxidizing microbes showed the highest consistency and sensitivity in response to the patch formation across the study sites. The abundance of amoA gene and archaeal amoA mRNA significantly increased in the degraded patches, and they were positively correlated with soil δ15N values and nitrate nitrogen contents, but negatively correlated with soil total nitrogen and inorganic nitrogen contents. These results suggest that the increased ammonia-oxidizing microbial abundance may be an important driver of soil nitrogen loss during degraded patch formation in alpine meadows.
View less >
View more >Alpine meadows on the Tibetan Plateau have experienced severe degradation in recent decades. Although the effects of alpine meadow degradation on soil properties have been well documented, there is still a paucity of knowledge regarding the responses of nitrogen-cycling microbes (NCMs) to degradation and their links to the changes in soil properties. Here, we systematically determined the effects of degraded patch formation on soil properties (i.e., total carbon, total nitrogen, ammonium nitrogen, nitrate nitrogen, available phosphorus, dissolved organic carbon, moisture, δ15N, δ13C, and pH) and NCMs (based on nifH, amoA, narG, nirK, and nirS genes and their transcripts) across three Tibetan alpine meadows at different degradation stages. Results showed that compared to the original grassed patches, the contents of most soil nutrients (e.g., carbon, nitrogen, and phosphorus) were significantly decreased in the degraded patches across the study sites. Degraded patches also tended to have higher soil δ15N values and nitrate contents. Among the aforementioned NCMs, soil diazotrophs and denitrifiers only showed weak responses to the patch formation, while ammonia-oxidizing microbes showed the highest consistency and sensitivity in response to the patch formation across the study sites. The abundance of amoA gene and archaeal amoA mRNA significantly increased in the degraded patches, and they were positively correlated with soil δ15N values and nitrate nitrogen contents, but negatively correlated with soil total nitrogen and inorganic nitrogen contents. These results suggest that the increased ammonia-oxidizing microbial abundance may be an important driver of soil nitrogen loss during degraded patch formation in alpine meadows.
View less >
Journal Title
Biogeochemistry
Volume
136
Issue
3
Subject
Other chemical sciences
Geochemistry
Soil biology
Environmental management