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dc.contributor.authorZhang, Li
dc.contributor.authorSriprakash, Kadaba S
dc.contributor.authorMcMillan, David
dc.contributor.authorGowardman, John R
dc.contributor.authorPatel, Bharat
dc.contributor.authorRickard, Claire M
dc.date.accessioned2017-05-03T15:25:05Z
dc.date.available2017-05-03T15:25:05Z
dc.date.issued2010
dc.date.modified2011-01-27T06:46:29Z
dc.identifier.issn1471-2180
dc.identifier.doi10.1186/1471-2180-10-266
dc.identifier.urihttp://hdl.handle.net/10072/35526
dc.description.abstractBackground: Intravascular catheter related infection (CRI) is one of the most serious nosocomial infections. Diagnostic criteria include a positive culture from the catheter tip along with blood, yet in many patients with signs of infection, current culture techniques fail to identify pathogens on catheter segments. We hypothesised that a molecular examination of the bacterial community on short term arterial catheters (ACs) would improve our understanding of the variety of organisms that are present in this niche environment and would help develop new methods for the diagnosis of CRI. Results: The whole bacterial community presenting on all ACs was evaluated by molecular methods, i.e., a strategy of whole community DNA extraction, PCR amplification followed by cloning and 16S rDNA sequence analysis. Ten ACs were removed from patients suspected of CRI and 430 clones from 5 "colonised" and 5 "uncolonised" (semiquantitative method) AC libraries were selected for sequencing and subsequent analysis. A total of 79 operational taxonomic units (OTUs) were identified at the level of 97% similarity belonging to six bacterial divisions. An average of 20 OTUs were present in each AC, irrespective of colonisation status. Conventional culture failed to reveal the majority of these bacteria. Conclusions: There was no significant difference in the bacterial diversity between the 'uncolonised' and 'colonised' ACs. This suggests that vascular devices cultured conventionally and reported as non infective may at times potentially be a significant source of sepsis in critically ill patients. Alternative methods may be required for the accurate diagnosis of CRI in critically ill patients.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.format.extent710452 bytes
dc.format.mimetypeapplication/pdf
dc.languageEnglish
dc.language.isoeng
dc.publisherBioMed Central
dc.publisher.placeUnited Kingdom
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom1
dc.relation.ispartofpageto9
dc.relation.ispartofjournalBMC Microbiology
dc.relation.ispartofvolume10
dc.rights.retentionY
dc.subject.fieldofresearchIntensive Care
dc.subject.fieldofresearchMedical Bacteriology
dc.subject.fieldofresearchInfectious Diseases
dc.subject.fieldofresearchBiological Sciences
dc.subject.fieldofresearchAgricultural and Veterinary Sciences
dc.subject.fieldofresearchMedical and Health Sciences
dc.subject.fieldofresearchcode110310
dc.subject.fieldofresearchcode110801
dc.subject.fieldofresearchcode110309
dc.subject.fieldofresearchcode06
dc.subject.fieldofresearchcode07
dc.subject.fieldofresearchcode11
dc.titleMicrobiological pattern of arterial catheters in the intensive care unit
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttp://creativecommons.org/licenses/by/2.0
gro.facultyGriffith Health, School of Nursing and Midwifery
gro.description.notepublicPage numbers are not for citation purposes. Instead, this article has the unique article number of 10:266
gro.rights.copyright© 2010 Zhang, et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
gro.date.issued2010
gro.hasfulltextFull Text
gro.griffith.authorPatel, Bharat K.
gro.griffith.authorZhang, Li
gro.griffith.authorMcMillan, David J.
gro.griffith.authorSriprakash, Kadaba S.
gro.griffith.authorRickard, Claire
gro.griffith.authorGowardman, John R.


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