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dc.contributor.authorZhang, Shanqingen_US
dc.contributor.authorZhao, Huijunen_US
dc.contributor.authorJohn, Richarden_US
dc.date.accessioned2017-04-24T10:42:38Z
dc.date.available2017-04-24T10:42:38Z
dc.date.issued2001en_US
dc.identifier.issn0003-2670en_US
dc.identifier.doi10.1016/S0003-2670(01)01079-0en_US
dc.identifier.urihttp://hdl.handle.net/10072/3554
dc.description.abstractA dual-phase amperometric biosensing device, incorporating immobilised tyrosinase, has been developed for the detection of phenolic compounds in both the aqueous and organic phases. The all-in-one biosensing probe, in which the electrodes, enzyme and buffer all reside behind a hydrophilic dialysis membrane, allowed direct analysis in the organic phase without the need for deliberately added electrolyte. The design also allowed analyses in both batch and flow injection measurement modes. In aqueous solutions, the unique configuration of the sensing device enabled the direct determination of 1 nM (0.1 姠l-1) phenol in batch mode and 10 nM (1 姠l-1) phenol in flow injection mode. Linearity of response over five orders of magnitude was also achieved. Relative responses for a range of phenolic compounds are also reported. In organic solvents, the sensitivity, linear range and response time were all shown to be very much dependent on the relative hydrophobicity of the solvents and substrates investigated. The effect of hydration for the immobilisation layer in the organic phase was also addressed.en_US
dc.description.peerreviewedYesen_US
dc.description.publicationstatusYesen_US
dc.languageEnglishen_US
dc.language.isoen_US
dc.publisherElsevier Scienceen_US
dc.publisher.placeNetherlandsen_US
dc.relation.ispartofpagefrom95en_US
dc.relation.ispartofpageto105en_US
dc.relation.ispartofjournalAnalytica Chimica Actaen_US
dc.relation.ispartofvolume441en_US
dc.subject.fieldofresearchcode250399en_US
dc.titleA dual-phase biosensing system for the determination of phenols in both aqueous and organic mediaen_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
gro.facultyGriffith Sciences, Griffith School of Environmenten_US
gro.date.issued2015-02-05T03:42:51Z
gro.hasfulltextNo Full Text


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