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dc.contributor.authorAnoopkumar-Dukie, Shailendraen_US
dc.contributor.authorGloyne, Leeen_US
dc.contributor.authorGrant, Garyen_US
dc.contributor.authorKiefel, Miltonen_US
dc.contributor.authorPerkins, Anthonyen_US
dc.contributor.editorDr. M. S. Ahmaden_US
dc.date.accessioned2017-04-04T19:54:28Z
dc.date.available2017-04-04T19:54:28Z
dc.date.issued2010en_US
dc.date.modified2011-07-12T07:52:18Z
dc.identifier.issn1948982Xen_US
dc.identifier.urihttp://hdl.handle.net/10072/35654
dc.description.abstractThis in vitro cell-based study identified the contributing role of pyocyanin in the resistance of Pseudomonas aeruginosa to ciprofloxacin. Problem statement: P. aeruginosa is the major pathogen in the Cystic Fibrosis (CF) lung with pyocyanin being a critical component of its virulence. Prevalence is high and, once acquired, chronic infection is difficult to eliminate. Ciprofloxacin remains a crucial oral agent effective against P. aeruginosa, but resistance is increasingly reported. Approach:Here we examined the extent to which exogenously added pyocyanin affected P. aeruginosa susceptibility to ciprofloxacin and the contribution of altered efflux activity and biofilm production with the aim of ultimately increasing sensitivity. Results: Metabolic conversion of resazurin to resorufin was used as an index of bacterial cell growth while fluorescent measurement of acriflavine efflux and crystal violet staining was used as markers of efflux activity and biofilm production, respectively. Pyocyanin (100 卩 added exogenously decreased susceptibility of two P. aeruginosa strains, PAO1 and ATCC 27853 to ciprofloxacin at 125 and 500 mg L-1, respectively. Exogenously added pyocyanin decreased efflux activity in both strains while biofilm production was significantly increased. Conclusion: We conclude that increased biofilm production may contribute to the observed decreased susceptibility of P. aeruginosa to ciprofloxacin. Ciprofloxacin is a crucial orally effectively agent against P. aeruginosa with resistance being increasingly reported. This initial study highlights a potential mechanism that may underlie this resistance which may be of clinical interest. Further studies using additional antibiotics and the pyocyanin precursor 1-hydroxyphenazine are required.en_US
dc.description.peerreviewedYesen_US
dc.description.publicationstatusYesen_AU
dc.format.extent112074 bytes
dc.format.mimetypeapplication/pdf
dc.languageEnglishen_US
dc.language.isoen_AU
dc.publisherScience Publicationsen_US
dc.publisher.placeUnited Statesen_US
dc.publisher.urihttp://www.thescipub.com/abstract/10.3844/ajmsp.2010.9.13en_AU
dc.relation.ispartofstudentpublicationYen_AU
dc.relation.ispartofpagefrom9en_US
dc.relation.ispartofpageto13en_US
dc.relation.ispartofissue1en_AU
dc.relation.ispartofjournalAmerican Journal of Microbiologyen_US
dc.relation.ispartofvolume1en_US
dc.rights.retentionYen_AU
dc.subject.fieldofresearchBasic Pharmacologyen_US
dc.subject.fieldofresearchcode111501en_US
dc.titleExogenous Pyocyanin Alters Pseudomonas aeruginosa Susceptibility to Ciprofloxacinen_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
gro.facultyGriffith Health, School of Pharmacyen_US
gro.rights.copyrightCopyright remains with the authors 2010. The attached file is reproduced here in accordance with the copyright policy of the publisher. For information about this journal please refer to the journal's website or contact the authors.en_AU
gro.date.issued2010
gro.hasfulltextFull Text


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