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dc.contributor.convenorMatthew Turnbull & David Palmeren_AU
dc.contributor.authorLove, Christopheren_US
dc.contributor.authorSolvander, Oscaren_US
dc.date.accessioned2017-05-03T11:37:22Z
dc.date.available2017-05-03T11:37:22Z
dc.date.issued2009en_US
dc.date.modified2011-01-31T05:53:23Z
dc.identifier.urihttp://hdl.handle.net/10072/35862
dc.description.abstractExpression of fX174 bacteriophage gpA and gpA* replication proteins: Internal promoters and toxic gene products. Solvander, O & Love, C.A. Eskitis Institue for Cell and Molecular Therapies & School of Biomolecular and Physical Sciences, Griffith University, Nathan, Qld, Australia. The faithful replication of DNA is pivotal for the survival of all organisms. However, small bacteriophage such as fX174 relies on its E. coli host for almost all its replication proteins. The only encoded proteins implicated in the replicating bacteriophage DNA are gene protein A (gpA) and gene protein A* (gpA*), and these proteins are involved in initiation of replication and shut-down of host DNA replication, respectively. Both proteins are encoded by the same gene and gpA* is expressed from an internal, in-frame ATG start codon. The resulting gpA* represents the C-terminal 341 residues of gpA and was reportedly translated from a polycistronic mRNA transcribed from a promoter located upstream of gene A. Attempts to clone gene A to produce quantities of gpA for structural and functional characterisation have been unsuccessful. Investigation of the 5' upstream region of gene A* revealed the presence of a putative promoter region, and if functional may allow the constitutive expression of gpA* during cloning, leading to the shut-down of host DNA replication which is lethal. We have shown that the placement of this putative region upstream of the luc gene resulted in the expression of luciferase confirming that the region acts as a promoter. The expression levels obtained from this promoter were twice that obtained using the lac promoter, and when inserted in the reverse orientation relative to the luc gene the expression levels were reduced by 98.6%.en_US
dc.description.publicationstatusYesen_AU
dc.format.extent89527 bytes
dc.format.mimetypeapplication/pdf
dc.languageEnglishen_US
dc.language.isoen_AU
dc.publisherAustralian Society for Biochemistry and Molecular Biologyen_US
dc.publisher.placeKent Town, SAen_US
dc.publisher.urihttp://www.asbmb.org.au/combio.htmlen_AU
dc.relation.ispartofstudentpublicationNen_AU
dc.relation.ispartofconferencenameCOMBIO2009 Conferenceen_US
dc.relation.ispartofconferencetitleProceedings of the Australian Society for Biochemistry and Molecular Biologyen_US
dc.relation.ispartofdatefrom2009-12-06en_US
dc.relation.ispartofdateto2009-12-10en_US
dc.relation.ispartoflocationChristchurch, NZen_US
dc.rights.retentionYen_AU
dc.subject.fieldofresearchBiochemistry and Cell Biology not elsewhere classifieden_US
dc.subject.fieldofresearchcode060199en_US
dc.titleExpression of φX174 bacteriophage gpA and gpA* replication proteins: Internal promoters and toxic gene products.en_US
dc.typeConference outputen_US
dc.type.descriptionE3 - Conference Publications (Extract Paper)en_US
dc.type.codeE - Conference Publicationsen_US
gro.rights.copyright© The Author(s) 2009. This is the author-manuscript version of this paper. It is posted here with permission of the copyright owner[s] for your personal use only. No further distribution permitted. For information about this Conference please refer to the Conference website or contact the authors.en_US
gro.date.issued2009
gro.hasfulltextFull Text


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