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  • Crystal structure of a fructokinase homolog from Halothermothrix orenii

    Author(s)
    Chua, Teck Khiang
    Seetharaman, J
    Kasprzak, Joanna M
    Ng, Cherlyn
    Patel, Bharat KC
    Love, Christopher
    Bujnicki, Janusz M
    Sivaraman, J
    Griffith University Author(s)
    Love, Christopher A.
    Year published
    2010
    Metadata
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    Abstract
    Fructokinase (FRK; EC 2.7.1.4) catalyzes the phosphorylation of d-fructose to d-fructose 6-phosphate (F6P). This irreversible and near rate-limiting step is a central and regulatory process in plants and bacteria, which channels fructose into a metabolically active state for glycolysis. Towards understanding the mechanism of FRK, here we report the crystal structure of a FRK homolog from a thermohalophilic bacterium Halothermothrix orenii (Hore_18220 in sequence databases). The structure of the Hore_18220 protein reveals a catalytic domain with a Rossmann-like fold and a ߭sheet "lid" for dimerization. Based on comparison of ...
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    Fructokinase (FRK; EC 2.7.1.4) catalyzes the phosphorylation of d-fructose to d-fructose 6-phosphate (F6P). This irreversible and near rate-limiting step is a central and regulatory process in plants and bacteria, which channels fructose into a metabolically active state for glycolysis. Towards understanding the mechanism of FRK, here we report the crystal structure of a FRK homolog from a thermohalophilic bacterium Halothermothrix orenii (Hore_18220 in sequence databases). The structure of the Hore_18220 protein reveals a catalytic domain with a Rossmann-like fold and a ߭sheet "lid" for dimerization. Based on comparison of Hore_18220 to structures of related proteins, we propose its mechanism of action, in which the lid serves to regulate access to the substrate binding sites. Close relationship of Hore_18220 and plant FRK enzymes allows us to propose a model for the structure and function of FRKs.
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    Journal Title
    Journal of Structural Biology
    Volume
    171
    Issue
    3
    DOI
    https://doi.org/10.1016/j.jsb.2010.05.007
    Subject
    Biochemistry and cell biology
    Biochemistry and cell biology not elsewhere classified
    Zoology
    Publication URI
    http://hdl.handle.net/10072/35946
    Collection
    • Journal articles

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