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  • Expression and localisation of thioredoxin during early implantation in the marmoset monkey

    Author(s)
    Lopata, A
    Sibson, MC
    Enders, AC
    Bloomfield, KL
    Gregory, MS
    Di Trapani, G
    Perkins, AV
    Tonissen, KF
    Clarke, FM
    Griffith University Author(s)
    Clarke, Francis M.
    Di Trapani, Jenny
    Gregory, Sarah-Jane S.
    Tonissen, Kathryn F.
    Year published
    2001
    Metadata
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    Abstract
    Thioredoxin is a powerful redox protein expressed in invasive cytotrophoblasts and essential for blastocyst implantation in mice. Isolated marmoset thioredoxin cDNA showed that the deduced amino acid sequence differed from the human sequence by four amino acids. The close homology of thioredoxin in the two species enabled us to use monoclonal antibodies against human thioredoxin to detect marmoset thioredoxin in implantation sites, blastocysts and culture medium. Immunocytochemistry on marmoset implantation sites, on pregnancy days 12 and 15, showed that thioredoxin is highly expressed in uterine luminal epithelium, glands ...
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    Thioredoxin is a powerful redox protein expressed in invasive cytotrophoblasts and essential for blastocyst implantation in mice. Isolated marmoset thioredoxin cDNA showed that the deduced amino acid sequence differed from the human sequence by four amino acids. The close homology of thioredoxin in the two species enabled us to use monoclonal antibodies against human thioredoxin to detect marmoset thioredoxin in implantation sites, blastocysts and culture medium. Immunocytochemistry on marmoset implantation sites, on pregnancy days 12 and 15, showed that thioredoxin is highly expressed in uterine luminal epithelium, glands and in some endometrial stromal cells. In attached blastocysts, thioredoxin staining was detected in mural and polar trophoblast cells and both visceral and parietal endoderm, whereas no staining was present in the inner cell mass. A similar pattern of thioredoxin expression was detected in hatched blastocysts attached to Matrigel in tissue culture. Trophoblastic vesicles derived from blastocysts expressed thioredoxin in inner endoderm-like cells and outer trophoblast-like cells and secreted thioredoxin into the culture medium. These experiments have demonstrated thioredoxin expression during early stages of embryo-maternal interaction. We propose that thioredoxin protects the early placenta from oxidative damage and that the marmoset is a valuable model for studying thioredoxin regulation and function during implantation and blastocyst differentiation.
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    Journal Title
    Molecular Human Reproduction
    Volume
    7
    Issue
    12
    Publisher URI
    http://molehr.oxfordjournals.org/cgi/content/full/7/12/1159
    http://molehr.oxfordjournals.org/cgi/content/abstract/7/12/1159
    Copyright Statement
    This is a pre-copy-editing, author-produced PDF of an article accepted for publication in [insert journal title] following peer review. The definitive publisher-authenticated version [insert complete citation information here] is available online at: http://molehr.oxfordjournals.org/cgi/reprint/7/12/1159
    Subject
    Zoology
    Clinical sciences
    History, heritage and archaeology
    Biochemistry and cell biology
    Reproductive medicine
    Publication URI
    http://hdl.handle.net/10072/3628
    Collection
    • Journal articles

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