Show simple item record

dc.contributor.authorWarrilow, David
dc.contributor.authorWarren, Kylie
dc.contributor.authorHarrich, David
dc.date.accessioned2017-05-03T11:48:31Z
dc.date.available2017-05-03T11:48:31Z
dc.date.issued2010
dc.date.modified2012-09-06T22:20:53Z
dc.identifier.issn19326203
dc.identifier.doi10.1371/journal.pone.0013229
dc.identifier.urihttp://hdl.handle.net/10072/36502
dc.description.abstractRecent work suggests a role for multiple host factors in facilitating HIV-1 reverse transcription. Previously, we identified a cellular activity which increases the efficiency of HIV-1 reverse transcription in vitro. Here, we describe aspects of the activity which shed light on its function. The cellular factor did not affect synthesis of strong-stop DNA but did improve downstream DNA synthesis. The stimulatory activity was isolated by gel filtration in a single fraction of the exclusion volume. Velocity-gradient purified HIV-1, which was free of detectable RNase activity, showed poor reverse transcription efficiency but was strongly stimulated by partially purified cell proteins. Hence, the cell factor(s) did not inactivate an RNase activity that might degrade the viral genomic RNA and block completion of reverse transcription. Instead, the cell factor(s) enhanced first strand transfer and synthesis of late reverse transcription suggesting it stabilized the reverse transcription complex. The factor did not affect lysis of HIV-1 by Triton X-100 in the endogenous reverse transcription (ERT) system, and ERT reactions with HIV-1 containing capsid mutations, which varied the biochemical stability of viral core structures and impeded reverse transcription in cells, showed no difference in the ability to be stimulated by the cell factor(s) suggesting a lack of involvement of the capsid in the in vitro assay. In addition, reverse transcription products were found to be resistant to exogenous DNase I activity when the active fraction was present in the ERT assay. These results indicate that the cell factor(s) may improve reverse transcription by facilitating DNA strand transfer and DNA synthesis. It also had a protective function for the reverse transcription products, but it is unclear if this is related to improved DNA synthesis.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.format.extent718487 bytes
dc.format.mimetypeapplication/pdf
dc.languageEnglish
dc.language.isoeng
dc.publisherPublic Library of Science
dc.publisher.placeUnited States
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom1
dc.relation.ispartofpageto9
dc.relation.ispartofissue10
dc.relation.ispartofjournalPloS One
dc.relation.ispartofvolume5
dc.rights.retentionY
dc.subject.fieldofresearchGenetics not elsewhere classified
dc.subject.fieldofresearchcode060499
dc.titleStrand Transfer and Elongation of HIV-1 Reverse Transcription Is Facilitated by Cell Factors In Vitro
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttp://www.plos.org/journals/license.html
gro.facultyGriffith Health, Griffith University Medical Research College
gro.rights.copyright© 2010 Warrilow et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution License CCAL. (http://www.plos.org/journals/license.html)
gro.date.issued2010
gro.hasfulltextFull Text
gro.griffith.authorHarrich, David
gro.griffith.authorWarrilow, David


Files in this item

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record