Crystallization and preliminary X-ray crystallographic analysis of zebrafish prototype galectin Drgal1-L2
Author(s)
Scott, Stacy A
Cozier, Matthew O
Dubar, Pauline DI
Ramakrishna, Manasa
Scott, Ken
Blanchard, Helen
Year published
2010
Metadata
Show full item recordAbstract
Zebrafish (Danio rerio) are an important developmental and embryological model given the optical clarity of embryos and larvae that permit real-time viewing of developing pathologies. More recently, a broader scope for these vertebrates to model a range of human diseases, including some cancers, has been indicated. Zebrafish Drgal1-L2 has been identified as an orthologue of mammalian galectin-1 that is a carbohydrate-binding protein exhibiting ߭galactoside binding specificity and which is over-expressed by many aggressive human cancers. This study describes the cloning, expression in Escherichia coli, purification and ...
View more >Zebrafish (Danio rerio) are an important developmental and embryological model given the optical clarity of embryos and larvae that permit real-time viewing of developing pathologies. More recently, a broader scope for these vertebrates to model a range of human diseases, including some cancers, has been indicated. Zebrafish Drgal1-L2 has been identified as an orthologue of mammalian galectin-1 that is a carbohydrate-binding protein exhibiting ߭galactoside binding specificity and which is over-expressed by many aggressive human cancers. This study describes the cloning, expression in Escherichia coli, purification and crystallisation of recombinant Drgal1-L2 protein in the presence of lactose (ligand). X-ray diffraction data from these novel crystals of zebrafish Drgal1-L2 were collected to a resolution of 1.5 Šusing a synchrotron radiation source, enabling their characterisation.
View less >
View more >Zebrafish (Danio rerio) are an important developmental and embryological model given the optical clarity of embryos and larvae that permit real-time viewing of developing pathologies. More recently, a broader scope for these vertebrates to model a range of human diseases, including some cancers, has been indicated. Zebrafish Drgal1-L2 has been identified as an orthologue of mammalian galectin-1 that is a carbohydrate-binding protein exhibiting ߭galactoside binding specificity and which is over-expressed by many aggressive human cancers. This study describes the cloning, expression in Escherichia coli, purification and crystallisation of recombinant Drgal1-L2 protein in the presence of lactose (ligand). X-ray diffraction data from these novel crystals of zebrafish Drgal1-L2 were collected to a resolution of 1.5 Šusing a synchrotron radiation source, enabling their characterisation.
View less >
Journal Title
Acta Crystallographica Section F
Volume
F66
Issue
12
Subject
Biological physics
Chemical sciences
Biological sciences