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dc.contributor.authorScott, Stacyen_US
dc.contributor.authorCozier, Matthewen_US
dc.contributor.authorDubar, Paulineen_US
dc.contributor.authorRamakrishna, Manasaen_US
dc.contributor.authorScott, Kenen_US
dc.contributor.authorBlanchard, Helenen_US
dc.contributor.editorEinspahr, Weissen_US
dc.date.accessioned2017-05-03T14:15:05Z
dc.date.available2017-05-03T14:15:05Z
dc.date.issued2010en_US
dc.date.modified2011-02-23T08:56:23Z
dc.identifier.issn17443091en_US
dc.identifier.doi10.1107/S1744309110042272en_AU
dc.identifier.urihttp://hdl.handle.net/10072/36511
dc.description.abstractZebrafish (Danio rerio) are an important developmental and embryological model given the optical clarity of embryos and larvae that permit real-time viewing of developing pathologies. More recently, a broader scope for these vertebrates to model a range of human diseases, including some cancers, has been indicated. Zebrafish Drgal1-L2 has been identified as an orthologue of mammalian galectin-1 that is a carbohydrate-binding protein exhibiting ߭galactoside binding specificity and which is over-expressed by many aggressive human cancers. This study describes the cloning, expression in Escherichia coli, purification and crystallisation of recombinant Drgal1-L2 protein in the presence of lactose (ligand). X-ray diffraction data from these novel crystals of zebrafish Drgal1-L2 were collected to a resolution of 1.5 Šusing a synchrotron radiation source, enabling their characterisation.en_US
dc.description.peerreviewedYesen_US
dc.description.publicationstatusYesen_AU
dc.languageEnglishen_US
dc.language.isoen_AU
dc.publisherWiley-Blackwell Publishing, Inc.en_US
dc.publisher.placeUnited Statesen_US
dc.relation.ispartofstudentpublicationNen_AU
dc.relation.ispartofpagefrom1647en_US
dc.relation.ispartofpageto1651en_US
dc.relation.ispartofissue12en_US
dc.relation.ispartofjournalActa Crystallographica Section Fen_US
dc.relation.ispartofvolumeF66en_US
dc.rights.retentionYen_AU
dc.subject.fieldofresearchBiological Physicsen_US
dc.subject.fieldofresearchcode029901en_US
dc.titleCrystallization and preliminary X-ray crystallographic analysis of zebrafish prototype galectin Drgal1-L2en_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
gro.facultyOffice of the Snr Dep Vice Chancellor, Institute for Glycomicsen_US
gro.date.issued2010
gro.hasfulltextNo Full Text


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