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dc.contributor.advisorQuinn, Ronald
dc.contributor.authorDashti, Yousef
dc.date.accessioned2018-01-23T02:20:49Z
dc.date.available2018-01-23T02:20:49Z
dc.date.issued2016
dc.identifier.doi10.25904/1912/1992
dc.identifier.urihttp://hdl.handle.net/10072/365648
dc.description.abstractSecondary metabolites and their derivatives from microorganisms are a source of many important drugs. Indeed almost half of the antibiotics in the market are actinomycetes sourced natural products. However, advances in genome sequencing have revealed that actinomycetes are capable of producing still a far greater number of metabolites and potential drugs. The machinery for making the majority of secondary metabolites is silent when actinomycetes are grown under standard culture conditions. Chapters 2 and 3 of this thesis focused on two different approaches to de- silence bacterial cryptic genes. A method involving 1H NMR fingerprinting was used to compare and investigate the metabolic profile of bacteria. This method is more comprehensive than the traditional analytical techniques such as LC-PDA and LC- MS. Metabolite profiling by LC-PDA and LC-MS is dependent either on the existence of a chromophore (PDA detection) or the ability of a compound to be ionised (MS detection) and might not detect all of the changes of the secondary metabolome. However, all organic compounds containing a proton are easily detected by NMR. Naturally microbes are in contact with different organisms. They produce secondary metabolites to communicate within or between species and also to defend themselves. One strategy to de-silent the cryptic genes is to mimic the environment and co-culture the microbes.
dc.languageEnglish
dc.publisherGriffith University
dc.publisher.placeBrisbane
dc.rights.copyrightThe author owns the copyright in this thesis, unless stated otherwise.
dc.subject.keywordsChemoinformatics
dc.subject.keywordsAnti-mycobacterial discovery
dc.subject.keywordsCryptic genome
dc.subject.keywordsNMR fingerprinting
dc.titleThe Cryptic Genome, NMR Fingerprinting and Chemoinformatics Towards Anti-mycobacterial Discovery
dc.typeGriffith thesis
gro.facultyScience, Environment, Engineering and Technology
gro.description.notepublicIn order to comply with copyright 2 articles have not been published here.
gro.rights.copyrightThe author owns the copyright in this thesis, unless stated otherwise.
gro.hasfulltextFull Text
dc.contributor.otheradvisorGrkovic, Tanja
dc.rights.accessRightsPublic
gro.identifier.gurtIDgu1480919916183
gro.thesis.degreelevelThesis (PhD Doctorate)
gro.thesis.degreeprogramDoctor of Philosophy (PhD)
gro.departmentSchool of Natural Sciences
gro.griffith.authorDashti, Yousef


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