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  • Genetic, Epigenetic and Morphological Evaluation of the Effects of Cryopreservation on Papaya

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    Kaity_2010_02Thesis.pdf (16.13Mb)
    Author(s)
    Kaity, Adam
    Primary Supervisor
    Drew, Rod
    Ashmore, Sarah
    Other Supervisors
    Panis, Jozefus
    Year published
    2010
    Metadata
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    Abstract
    A vitrification-based cryopreservation technique for storage of in vitro shoot tips of papaya has been tested to ensure applicability across a range of genotypes and to assess the stability of both genotype and phenotype of clonal material following cryopreservation. Shoot tips of twelve female genotypes were cryopreserved, regeneration percentages were determined and resultant plants were screened for genetic and epigenetic changes. Genomic DNA structure was studied using polymerase chain reaction (PCR) based randomly amplified DNA fingerprinting (RAF), and methylation patterns were monitored using the amplified ...
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    A vitrification-based cryopreservation technique for storage of in vitro shoot tips of papaya has been tested to ensure applicability across a range of genotypes and to assess the stability of both genotype and phenotype of clonal material following cryopreservation. Shoot tips of twelve female genotypes were cryopreserved, regeneration percentages were determined and resultant plants were screened for genetic and epigenetic changes. Genomic DNA structure was studied using polymerase chain reaction (PCR) based randomly amplified DNA fingerprinting (RAF), and methylation patterns were monitored using the amplified DNA methylation polymorphism (AMP) PCR technique. Plantlets were regenerated following cryopreservation in all but one genotype and regeneration percentages of 61-73% were obtained from 6 genotypes. The regenerated plantlets showed varying levels of genomic DNA modifications (0-10.07%), and methylation modifications (0.52-6.62%) for detected markers. These findings have not been reported previously for papaya, and indicate some genotype dependent variability in DNA modifications occurred following cryopreservation which could result in somaclonal variation.
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    Thesis Type
    Thesis (PhD Doctorate)
    Degree Program
    Doctor of Philosophy (PhD)
    School
    School of Biomolecular and Physical Sciences
    DOI
    https://doi.org/10.25904/1912/211
    Copyright Statement
    The author owns the copyright in this thesis, unless stated otherwise.
    Item Access Status
    Public
    Subject
    Cryopreservation
    Papaya cells
    Cryopreservation of papaya shoot tips
    Publication URI
    http://hdl.handle.net/10072/365796
    Collection
    • Theses - Higher Degree by Research

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