Cytotoxicity Screening of Bangladeshi Medicinal Plants and Isolation and structural Elucidation of Novel Anti-Cancer Compounds from Acrostichum aureum
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Author(s)
Primary Supervisor
Tiralongo, Evelin
Other Supervisors
Grice, Darren
Jason, Tracey
Year published
2011
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This thesis describes the cytotoxicity and phytochemical screening of 16 selected medicinal plants collected from the tidal forests of the coastal Sundarban and locations within the Khulna district of Bangladesh. The selected plants were successively extracted with n-hexane, dichloromethane, methanol and water. The methanol and aqueous extracts were then screened for cytotoxic activity (MTT assay) against one normal mouse fibroblast and three human cancer cell lines (gastric, colon and breast cancer). The results supported some of the traditional uses and reported cytotoxic activity for some of these plants. It identified ...
View more >This thesis describes the cytotoxicity and phytochemical screening of 16 selected medicinal plants collected from the tidal forests of the coastal Sundarban and locations within the Khulna district of Bangladesh. The selected plants were successively extracted with n-hexane, dichloromethane, methanol and water. The methanol and aqueous extracts were then screened for cytotoxic activity (MTT assay) against one normal mouse fibroblast and three human cancer cell lines (gastric, colon and breast cancer). The results supported some of the traditional uses and reported cytotoxic activity for some of these plants. It identified some plant extracts (Hygrophila auriculata, Hibiscus tiliaceous and Limnophila indica) with selective cytotoxic activity to cancer cells (IC50 1.1 - 1.6 mg/mL) but non-toxic to normal mouse fibroblast cells. Some extracts (Acrostichum aureum, Ammannia baccifera, Argemone mexicana, Limnophila indica, Clerodendron inerme, Cynometra ramiflora and Xylocarpus moluccensis) showed low cytotoxicity against mouse fibroblast (IC50 > 2.5 mg/mL) but selective potent toxicity against cancer cells (IC50 0.2 - 2.3 mg/mL). Four extracts (Adiantum caudatum, Hibiscus tiliaceous and Blumea lacera) showed cytotoxic activity against all the cell lines tested in the study. The methanolic and aqueous extracts of B. lacera were identified as having the most potent cytotoxic activity against all cell lines with IC50 0.01 - 0.08 mg/mL. Reversed-phase analytical HPLC profiling of all extracts was carried out with extracts, demonstrating low toxicity against normal mouse fibroblast cells, but selective high toxicity against the three cancerous cell lines (A. aureum and A. baccifera) and highest toxicity against all the cell lines (B. lacera). Extracts from these plants were further investigated by LC-MS. A. aureum was then selected and further investigated with one compound subsequently being isolated from n-hexane fraction and twelve compounds from the methanolic fraction. Three compounds were identified as novel natural products, namely (2'Smethylhexyl) (2''S-methyl-5''-acetylpentyl) phthalate (1), (2R, 3S)-sulfated pterosin C (2) and (2S, 3S)-sulfated pterosin C (5).
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View more >This thesis describes the cytotoxicity and phytochemical screening of 16 selected medicinal plants collected from the tidal forests of the coastal Sundarban and locations within the Khulna district of Bangladesh. The selected plants were successively extracted with n-hexane, dichloromethane, methanol and water. The methanol and aqueous extracts were then screened for cytotoxic activity (MTT assay) against one normal mouse fibroblast and three human cancer cell lines (gastric, colon and breast cancer). The results supported some of the traditional uses and reported cytotoxic activity for some of these plants. It identified some plant extracts (Hygrophila auriculata, Hibiscus tiliaceous and Limnophila indica) with selective cytotoxic activity to cancer cells (IC50 1.1 - 1.6 mg/mL) but non-toxic to normal mouse fibroblast cells. Some extracts (Acrostichum aureum, Ammannia baccifera, Argemone mexicana, Limnophila indica, Clerodendron inerme, Cynometra ramiflora and Xylocarpus moluccensis) showed low cytotoxicity against mouse fibroblast (IC50 > 2.5 mg/mL) but selective potent toxicity against cancer cells (IC50 0.2 - 2.3 mg/mL). Four extracts (Adiantum caudatum, Hibiscus tiliaceous and Blumea lacera) showed cytotoxic activity against all the cell lines tested in the study. The methanolic and aqueous extracts of B. lacera were identified as having the most potent cytotoxic activity against all cell lines with IC50 0.01 - 0.08 mg/mL. Reversed-phase analytical HPLC profiling of all extracts was carried out with extracts, demonstrating low toxicity against normal mouse fibroblast cells, but selective high toxicity against the three cancerous cell lines (A. aureum and A. baccifera) and highest toxicity against all the cell lines (B. lacera). Extracts from these plants were further investigated by LC-MS. A. aureum was then selected and further investigated with one compound subsequently being isolated from n-hexane fraction and twelve compounds from the methanolic fraction. Three compounds were identified as novel natural products, namely (2'Smethylhexyl) (2''S-methyl-5''-acetylpentyl) phthalate (1), (2R, 3S)-sulfated pterosin C (2) and (2S, 3S)-sulfated pterosin C (5).
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Thesis Type
Thesis (PhD Doctorate)
Degree Program
Doctor of Philosophy (PhD)
School
School of Pharmacy
Copyright Statement
The author owns the copyright in this thesis, unless stated otherwise.
Item Access Status
Public
Subject
Medicinal plants
Cytotoxicity
Phytochemical screening
Khulna district Bangladesh
Novel anti-cancer compounds
Acrostichum aureum