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dc.contributor.advisorUlett, Glen
dc.contributor.authorTan, Chee Keong
dc.date.accessioned2018-01-23T02:25:51Z
dc.date.available2018-01-23T02:25:51Z
dc.date.issued2013
dc.identifier.doi10.25904/1912/2418
dc.identifier.urihttp://hdl.handle.net/10072/366082
dc.description.abstractUrinary tract infection (UTI) is one of the most common infections and costs millions of dollar each year to the health care industry. Escherichia coli is the causative agent responsible for over 80% of UTIs. However, other pathogens such as Group B streptococcus (GBS), a Grampositive bacterium, also cause UTI. In addition, GBS is an important commensal microbe in the female genital tract. Researchers have used various murine models to study mechanisms of disease pathogenesis for UTI and microbe colonisation of the genital tract. However, there is a lack of understanding of the pathogenic mechanisms and microbe virulence factors involved in UTI and genital tract colonisation due to both E. coli and GBS. This thesis reports on a series of experiments using murine models to better understand the pathogenesis of E. coli and GBS infection in the urogenital tract. It explores the use of several murine models to better understand host responses to these infections. In one series of experiments on both E. coli and GBS, murine models were utilised to define the global host immune response to these bacteria in UTI on a genome-wide scale with the aid of microarrays. Another series of experiments used a murine model of E. coli UTI to study the virulence factor [alpha]-hemolysin and how this factor influences host responses. The thesis also describes the development of a novel GBS long-term genital tract colonisation model in mice that was used to define the nature of GBS colonisation and survival in this infection. Thus, a series of novel murine models are described in the thesis that were applied in various host response studies and virulence assays to better understand how E. coli and GBS survive and cause infection in the urogenital tract in vivo. The application of murine models to study urogenital tract infection as described in this thesis provides researchers with a solid platform to examine the effects of virulence factors, and host responses in more detail in future studies. One particular area for future research relates to the specific series of experiments in which a murine UTI model was used to test the efficacy of prophylactic use of a novel E. coli 83972 'probiotic strain' expressing a P fimbriae oligosaccharide receptor mimic to prevent acute infection. In this way, the data described in this thesis also provides vital new insight into how murine models can be used to test possible novel treatment strategies for preventing UTI.
dc.languageEnglish
dc.publisherGriffith University
dc.publisher.placeBrisbane
dc.rights.copyrightThe author owns the copyright in this thesis, unless stated otherwise.
dc.subject.keywordsUrinary tract infection (UTI)
dc.subject.keywordsEscherichia coli
dc.subject.keywordsGroup B streptococcus (GBS)
dc.subject.keywordsStreptococcus Agalactiae
dc.subject.keywordsMurine Colonisation Models
dc.subject.keywordsFemale Urogenital Tract
dc.titleDevelopment and Application of Escherichia coli and Streptococcus Agalactiae Murine Colonisation Models to Study Bacterial Pathogenesis in the Female Urogenital Tract
dc.typeGriffith thesis
gro.facultyGriffith Health
gro.description.notepublicIn order to comply with copyright some articles have not been published here.
gro.rights.copyrightThe author owns the copyright in this thesis, unless stated otherwise.
gro.hasfulltextFull Text
dc.contributor.otheradvisorCripps, Allan
dc.contributor.otheradvisorCarey, Alison
dc.rights.accessRightsPublic
gro.identifier.gurtIDgu1424404228833
gro.source.ADTshelfnoADT0
gro.source.GURTshelfnoGURT
gro.thesis.degreelevelThesis (PhD Doctorate)
gro.thesis.degreeprogramDoctor of Philosophy (PhD)
gro.departmentSchool of Medical Science
gro.griffith.authorTan, CK K.


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