Effects of Sensory Ablation on the Young and Aged Ventriculo-Olfactory Neurogenic System
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Adult neurogenesis is a dynamic field of study, with potential for generating therapies for neurodegenerative disorders. The demonstration of adult brain neurogenesis strengthens the rationale for regenerative therapies for neurodegenerative disorders. The ‘ventriculo-olfactory neurogenic system’ (VONS) is used in the study neurogenesis, migration and differentiation of neural stem cells. Importantly, subventricular zone (SVZ) neuroblasts recapitulate a population of dopaminergic neurons within the olfactory bulb (OB), possibly the only population of dopaminergic interneurons, generated throughout adult life. This study aims to simulate a patho physiological model for olfactory epithelial cell turnover to demonstrate a link between the olfactory epithelium (OE) and cell proliferation in the SVZ. In order to examine the relationship between the OE and the SVZ, an ablation protocol was generated. Using a double dose of methimazole, the OE was completely ablated leading to denervation of the olfactory bulb. This denervation resulted in an increase of astrocyte and microglial activity on day 11 which remained elevated up to day 21. This was accompanied by a significant decrease of tyrosine hydroxylase immunoreactivity in type I periglomerular cells on day 17. While there was a decrease in the type II periglomerular cells on day 17, this was shown to be not significant.
Thesis (PhD Doctorate)
Doctor of Philosophy (PhD)
School of Biomolecular and Physical Sciences
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Ventriculo-olfactory neurogenic system (VONS)